Antibodies & immunoassays for small molecules Serotonin Antibody – Mouse Monoclonal
Ref: IS040
NeuromediatorsTryptophan metabolismMonoclonal AntibodiesICCIFIHC
+Serotonin / Tyrosine Hydroxylase in mouse embryonic midbrain
Immunodetection of serotonin (red – IS040) and Tyrosine hydroxylase (green) in the mouse embryonic midbrain (E13.5) using mouse monoclonal anti-serotonin antibody (IS040) and rabbit polyclonal anti-TH antibody, following protocol for whole mount samples provided with STAINPerfect kit A (SP-A-1000).
DatasheetMSDS
Anti-Serotonin antibody IS040 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels 5-HT in whole mounts, cell cultures and tissue sections.
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Applications | IHC / IF |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL |
| References | Cited in 2 papers |
Product overview
| Product name | Serotonin antibody – mouse mAb |
| Synonyms | Anti-5-HT antibody
Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A.Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Serotonin staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Product citations
- INSIHGT: Accessible multimodal systems biology with quantitative molecular phenotyping in 3D
Check the article
Authors : Chun et al., bioRxiv
2024-06 - Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
Check the article
Authors : Smits et al., Cell & tissue research
2020-07
