Antibodies & immunoassays for small molecules Serotonin Monoclonal Antibody
Ref: IS040
NeuromediatorsTryptophan metabolismMonoclonal AntibodiesICCIFIHC
+Serotonin / Tyrosine Hydroxylase in mouse embryonic midbrain
Immunodetection of serotonin (red – IS040) and Tyrosine hydroxylase (green) in the mouse embryonic midbrain (E13.5) using mouse monoclonal anti-serotonin antibody (IS040) and rabbit polyclonal anti-TH antibody, following protocol for whole mount samples provided with STAINPerfect kit A (SP-A-1000).
DatasheetMSDS
Anti-Serotonin antibody IS040 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels 5-HT in whole mounts, cell culture and tissue sections.
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Applications | IHC / IF |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL |
Product overview
| Product name | Serotonin antibody – mouse mAb |
| Synonyms | Anti-5-HT antibody
Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A.Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Serotonin staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Antibody not yet cited. Submit an article and get a 10% discount.
Selected papers about serotonin:
- Gershon MD. 5-Hydroxytryptamine (serotonin) in the gastrointestinal tract. Curr Opin Endocrinol Diabetes Obes. 2013 Feb;20(1):14-21. doi: 10.1097/MED.0b013e32835bc703.
- Baganz NL, Blakely RD. A dialogue between the immune system and brain, spoken in the language of serotonin. ACS Chem Neurosci. 2013 Jan 16;4(1):48-63. doi: 10.1021/cn300186b. Epub 2012 Dec 7.
- Bortolato M et al. The role of the serotonergic system at the interface of aggression and suicide. Neuroscience. 2013 Apr 16;236:160-85.
- Kiyasova V et al. A genetically defined morphologically and functionally unique subset of 5-HT neurons in the mouse raphe nuclei. J Neurosci. 2011 Feb 23;31(8):2756-68. doi: 10.1523/JNEUROSCI.4080-10.2011.
