Serotonin Monoclonal AntibodyRef: IS040
Anti-Serotonin antibody IS040 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels 5-HT in whole mounts, cell culture and tissue sections.
|Applications||IHC / IF|
|Reactivity||Reacts with all species|
|Tested samples||Whole mounts, cell culture, tissue sections|
|Staining procedure||STAINperfect immunostaining kit A|
|Product name||Serotonin antibody – mouse mAb|
|Specificity||When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine|
|Storage||Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles|
|Material safety datasheet||Download MSDS|
|IF – Cell cultures, Whole mounts, Tissue sections||Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A.Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample|
|Comments||Optimal working dilutions must be determined by the end-user|
|Restrictions||For research use only|
|Full protocol||Download STAINperfect protocol for Serotonin staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
Antibody not yet cited. Submit an article and get a 10% discount.
Selected papers about serotonin:
- Gershon MD. 5-Hydroxytryptamine (serotonin) in the gastrointestinal tract. Curr Opin Endocrinol Diabetes Obes. 2013 Feb;20(1):14-21. doi: 10.1097/MED.0b013e32835bc703.
- Baganz NL, Blakely RD. A dialogue between the immune system and brain, spoken in the language of serotonin. ACS Chem Neurosci. 2013 Jan 16;4(1):48-63. doi: 10.1021/cn300186b. Epub 2012 Dec 7.
- Bortolato M et al. The role of the serotonergic system at the interface of aggression and suicide. Neuroscience. 2013 Apr 16;236:160-85.
- Kiyasova V et al. A genetically defined morphologically and functionally unique subset of 5-HT neurons in the mouse raphe nuclei. J Neurosci. 2011 Feb 23;31(8):2756-68. doi: 10.1523/JNEUROSCI.4080-10.2011.