Antibodies & immunoassays for small molecules Serotonin Polyclonal Antibody
Ref: IS1086
NeuromediatorsTryptophan metabolismPolyclonal AntibodiesIFIHC
+Immunohistochemistry against serotonin (5HT) on a FFPE human colon coronal section
5HT immunoreactivity, revealed using anti-serotonin (5HT, rabbit polyclonal antibody #IS1086), is highlighted in enterochromaffin cells of the intestinal mucosa.
DatasheetMSDS
This novel antibody raised against Serotonin (5-hydroxytryptamine, 5-HT) (#IS1086, polyclonal) is compatible with formalin-fixed biological materials. While allowing for the specific immunodetection of the serotonergic systems, our antibody has been validated for its use through immunochemistry and immunofluorescence applications on formalin-fixed paraffin-embedded (FFPE) samples originating from different species.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Validated applications |
IF/IHC-P, IF/IHC-F (manual and automated staining) |
| Format | 50µL |
Product overview
| Product name | Serotonin antibody (5-HT rabbit polyclonal Ab) |
| Synonyms | Anti-5-HT antibody
Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated serotonin antibody did not cross-react with 5-hydroxytryptamine analogs, including tryptamine and 5-hydroxytryptophan |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute at 1/250 -1/1000 |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
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