The clear path to small molecule visualization & quantification

Key features

Sample type 10µL urine, 25µL plasma
Capacity 96-well plate
Sensitivity Urine: 0.3 ng/mL
Plasma: 0.19 ng/mL
Range 0/0.5 – 50 ng/mL
Assay time 5h
Reactivity Reacts with all species
Application In vitro diagnostics (IVD in EU only), or Research use

Citations

Frequently asked questions

BA-E-1000

Histamine ELISA kit – Plasma & Urine

This Histamine ELISA kit is tailored for the quantitative measurement of histamine in urine and plasma samples. Intended for in vitro diagnostics or research use, the immunoassay can be combined with the add-on kit BA-E-1100, to quantify histamine release in whole blood.

399

Number of citations :
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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online. 

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BA-E-1000

Histamine ELISA kit – Plasma & Urine

Histamine ELISA kit – Research test for the detection of histamine

Specifications

Product name Histamine ELISA kit – Urine and Plasma samples
Description In vitro diagnostics & research enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in urine and plasma samples
Format  96-well plate
Samples Urine & Plasma samples
Minimal sample volume 10µL urine, 25µL plasma
Reactivity Reacts with all species
Standard range 0/0.5 – 50 ng/mL
Sensitivity

Urine: 0.3 ng/mL
Plasma: 0.19 ng/mL

Specificity No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin
Assay time 5 hours

Instructions

Storage  Store at 2-8°C for to 6 months
Sample collection & storage Plasma: EDTA or Heparin plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C
Sample preparation Sample acylation (1h)
ELISA Histamine antiserum incubation (3h), revelation and read steps (1h)

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