Histamine release add-on kit Histamine release add-on kit

Histamine release add-on kit

Histamine release kit (add-on) - Whole blood samples

Ref: BA-E-1100

 

This add-on kit to the BA-E-1000 histamine ELISA kit enables the measurement of histamine release in heparinized whole blood samples, allowing for instance to determine the sensitivity of basophilic granulocytes to specific allergens.

 

Sample type
500 µl whole blood
Samples/kit 40 samples in duplicate
Sensitivity

0.2 ng/ml

Range 0 / 0.5 - 50 ng/ml
Assay time

Sample preparation (1h30)

Species reactivity Reacts with all species
Citations 3
70
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(for 1 kit)
54.00€ , 5 days
Product information Key steps References Technical support Reviews

Product overview

Product name

Histamine release kit - Whole blood samples

Description Supplementary kit to in vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine release in whole blood samples.
Labels IVD, CE
Format 96-well plate
Samples 500 µl whole blood
Cross specie-reactivity React with all species
Standard range 0 / 0.5 - 50 ng/ml
Sensitivity

0.2 ng/ml

Specificity

No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin

Assay time 1h30 sample preparation + ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for use Material safety datasheet
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Key steps

Sample collection & storage

24-hours heparinized whole blood stored at 20-25°C.

Sample preparation

Sample acylation (1h30)

ELISA Histamine antiserum incubation (3H or overnight), revelation and read steps (1h).
Detailed protocol Download instructions for use
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