Histamine ELISA kit
High Sensitivity I Plasma & Urine

Ref: BA-E-1000
NeuromediatorsHistamineDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
DatasheetMSDSProtocol

This Histamine ELISA kit is tailored for the quantitative measurement of histamine in urine and plasma samples. Intended for in vitro diagnostics & research, the immunoassay can be combined with the add-on kit BA-E-1100, to quantify histamine release in whole blood.

Sample type10µl urine, 25µl plasma
Samples/kit40 samples in duplicate
Sensitivity0.3ng/ml urine, 0.12ng/ml plasma
Range0.5 – 50ng/ml
Assay timeSample acylation (1h) and ELISA overnight
ReactivityReacts with all species
CitationsCited in 5 papers
Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

340.00

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Quantity
Total

340.00

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Product overview

Product nameHistamine ELISA kit – Urine and Plasma samples
DescriptionIn vitro diagnostics & research enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in urine and plasma samples
LabelsIVD, CE
Format96-well plate
Samples10µl urine, 25µl plasma
ReactivityReacts with all species
Standard range0 / 0.5 – 50ng/ml
Sensitivity

0.12ng/ml plasma, 0.3ng/ml urine

SpecificityNo significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin
Assay time1h sample acetylation + ELISA overnight
StorageStore at 2-8°C for up to 6 months
DatasheetsInstructions for useMaterial safety datasheet

 

Sample collection & storagePlasma: EDTA or Heparin plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C

Sample preparationSample acylation (1h)
ELISAHistamine antiserum incubation (3H or overnight), revelation and read steps (1h)
Detailed protocolDownload instructions for use

Product citation:

 

  • Increased serum diamine oxidase activity in nonallergic patients with migraine
    Check the article
    Authors : Garcia-Martin et al, European journal of Clinical Investigation
    2022-02
  • Inactivated antithombin as anticoagulant reversal in a rat model of cardiopulmonary bypass: a potent and potentially safer alternative to protamine
    Check the article
    Authors : Bianchini et al., Britis journal of Haematology
    2018-01
  • TCTP/HRF pathway and angiogenesis: a feasible intercourse in chronic lymphocytic leukemia
    Check the article
    Authors : Yagcı et al., Leukemia Research
    2013-02
  • The effect of the extract of Crocus sativus and its constituent safranal, on lung pathology and lung inflammation of ovalbumin sensitized guinea-pigs
    Check the article
    Authors : Boskabady et al., Photomedicine
    2012-06
  • H2-receptor-mediated vasodilation contributes to postexercise hypotension
    Check the article
    Authors : McCord et al., Journal of Applied Physiology
    2005-09

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