Antibodies & immunoassays for small molecules Kynurenic Acid ELISA kit
Ref: IS-I-0200R
+Cross-validation of Kynurenic Acid ELISA and LC/MS data in human serum samples
Human serum samples (n=40) were processed for Kynurenic Acid measurement by mean of both KYNA ELISA kit and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS are highly correlated (R Squared=0,9902).
Kynurenic acid (KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to possess several neuromodulatory functions, as well as immuno-modulatory properties. Measurement of KYNA in biological samples has been presented as a possible biomarker in several diseases including neurological or psychiatric disorders.
The Kynurenic Acid ELISA kit is optimized for the quantitation of Kynurenic Acid (KYNA) within serum samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a 50µL sample volume, this assay enables pre-clinical and clinical sample testings.
| Sample type | Serum |
| Capacity | 96 tests |
| Sensitivity | 2.8nM |
| Range | 10 – 391nM |
| Assay time | Sample derivatization 90 min, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | Kynurenic Acid ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Kynurenic Acid in serum samples. For research use only |
| Format | 96-well plate |
| Samples | Serum |
| Minimal sample volume | 50µL |
| Reactivity | Reacts with all species |
| Standard range | 1.40 – 74ng/mL |
| Sensitivity |
0.53ng/mL |
| Specificity | No significant cross-reactivity was observed with Kynurenic Acid analogs such as Quinolinic Acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3-Hydroxy-Anthranilic Acid |
| Assay time | Sample derivatization 90 min and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample derivatization (90 min) |
| ELISA | Kynurenic acid antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Alzheimer’s-like signaling in brains of COVID-19 patients
Check the article
Authors : Reiken et al., Alzheimer's & Dementia
2022-02
