L-Glutamine ELISA kit

Ref: IS-I-1100R
Amino acidsResearch ELISA kits (RUO)ELISA
DatasheetMSDSProtocol

Glutamine (Gln) is an abundant non-essential amino acid that is involved in several physiological functions including the regulation of the immune system . However, in some disease conditions, Gln metabolism can be highly dysregulated. In cancer, Gln is highly consumed by tumor cells as to fulfill their bioenergetic and biosynthetic requirements for proliferation. Targeting Gln catabolism – mainly Glutaminase – is currently investigated as a therapeutic approach to circumvent high Gln consumption by cancer cells and maintain a sufficient level in the microenvironment for an effective anti-tumor immune response.

The IS-I-1100 Gln ELISA kit allows for the determination of L-Glutamine in plasma and serum, originating from either preclinical or clinical samples, of a volume as low as 10μL and with a sensitivity of 3µM.

Sample type Plasma, Serum, Cell supernatant
Capacity 96 tests
Sensitivity 3µM
Range 21 – 3125µM
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species

 

Size

1 x 96 wells

490.00

100 in stock

Quantity
Total

490.00

Worldwide shipping

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Product overview

Product name Glutamine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Glutamine (Gln) in plasma and serum samples. For research use only
Format 96-well plate
Samples Plasma, Serum, Cell supernatant
Minimal sample volume 10µL
Reactivity Reacts with all species
Standard range 21 – 3125µM
Sensitivity

3µM

Specificity No significant cross-reactivity was observed with Ornithine, Arginine, Asparagine, D-Glutamine and L-Glutamic acid
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storage EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA L-Glutamine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

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