Antibodies & immunoassays for small molecules L-Kynurenine Monoclonal Antibody
Ref: IS003
Kynurenine pathwayMonoclonal AntibodiesICCIFIHC
+L-Kynurenine visualization in human intestinal immune cells by IF
Immunofluorescence staining reveals L-Kynurenine accumulation in specific immune cells in human colon tissue. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/250). After incubation with Alexa-488 conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize IF staining.
DatasheetMSDS
Monoclonal anti-L-Kynurenine antibody 3D4-F2 enabled to illustrate, for the first time, the presence of L-kynurenine in human brain and tumor samples by IHC and IF. Recently, our anti-kynurenine antibody was used to stratify cancer patients for kynurenine pathway activity (open-access PLOS ONE article).
| Clonality | Monoclonal antibody (clone 3D4-F2) |
| Host | Mouse |
| Validated applications | IHC / IF |
| Reactivity | Reacts with all species |
| References | 5 citations |
| Format | 50µL |
Product overview
| Product name | L-Kynurenine antibody |
| Synonyms | (S)-Kynurenine antibody
L-2-Amino-4-(2-aminophenyl)-4-oxobutanoic acid antibody Kynurenin antibody 3-Anthraniloyl-L-alanine antibody, |
| Immunogen | Conjugated L-Kynurenine |
| Isotype | IgG1 k chain |
| Clone | clone 3D4-F2 |
| Specificity | When tested in competitive ELISA, the anti-L-Kynurenine antibody did not show any significant cross reactivity with L-Tryptophan, 3-hydroxy-DL-Kynurenine, Kynurenic acid, Anthranilic acid or 3-hydroxyAnthranilic acid conjugates |
Storage
| Form | Liquid |
| Purity | Purified IgG |
| Concentration | 0,5mg/ml |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| Immunohistochemistry (IHC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) | Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
