L-Tryptophan ELISA kit
Any sample I High Sensitivity

Ref: BA-E-2700
Tryptophan metabolismAmino acidsDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
DatasheetMSDSIVD ProtocolRUO Protocol

This Tryptophan ELISA kit enables the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Intended for in vitro diagnotics and research applications, the test comes in 96-well plate format, allowing the screening of large experimental series within short lead times.

Sample type Biological fluids (urine, plasma, serum, …) and cell culture supernatant
Capacity 96 tests
Sensitivity < 1.2µg/ml
Range 2.5 – 250µg/ml
Assay time 2h30 sample preparation and ELISA overnight
Reactivity Reacts with all species
References Cited in >40 papers

 

Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

453.00

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Quantity
Total

453.00

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Product overview

Product name Tryptophan ELISA kit
Description In vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, …) and cell culture supernatants
Labels IVD, CE
Format 96-well plate
Samples urine, plasma, serum, cell culture supernatant
Sample volume 20µL
Reactivity Reacts with all species
Standard range 2.5 – 250µg/ml
Sensitivity

< 1.2µg/ml

Specificity No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine
Assay time Sample preparation 2.5 hours and ELISA overnight
Storage Store at 2-8°C for to 6 months
Datasheets Instructions for use Material safety datasheet

 

Sample collection & storage Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)

Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples

Sample preparation Sample precipitation (15 min) and derivatization (2h15)
ELISA Tryptophan antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol IVD: Download instructions for use

RUO: Download instructions for use

Product citation

  • Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy
    Check the article
    Authors : Wang et al., Nature Communications
    2023-11
  • Dietary Marine Hydrolysate Improves Memory Performance and Social Behavior through Gut Microbiota Remodeling during Aging
    Check the article
    Authors : Mougin et al., Foods
    2023-11
  • An open label randomized controlled trial of atorvastatin versus aspirin in elite controllers and antiretroviral-treated people with HIV
    Check the article
    Authors : Mystakeli et al., AIDS
    2023-10
  • Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress
    Check article
    Authors : Letenneur et al., Nutrients
    2023-10
  • Hypomethylation-induced regulatory programs in T cells unveiled by transcriptomic analyses
    Check the article
    Authors : Lysandrou et al., Frontiers in Immunology
    2023-09
  • Pathogenetic associations of anti-ribosomal P protein antibody titres and their subclasses in patients with systemic lupus erythematosus
    Check the article 
    Authors : Kaneko et al., Rheumatology
    2023-08
  • Tryptophan Modulation in Cancer-Associated Cachexia
    Check the article
    Authors : Agullo-Ortuno et al., International Journal of Molecular Sciences
    2023-08
  • Intake of Lactiplantibacillus plantarum HEAL9 Improves Cognition in Moderately Stressed Subjects: A Randomized Controlled Study
    Check the article
    Authors : Onning et al., Nutrients
    2023-08
  • Effects of Supplementation with the Standardized Extract of Saffron (affron®) on the Kynurenine Pathway and Melatonin Synthesis in Rats
    Check the article
    Authors : Munoz et al., Antioxydants
    2023-07
  • Advances in kynurenine analysis
    Check the article
    Authors : Mrstna et al., Clinica Chimica Acta
    2023-07
  • Involvement of the kynurenine pathway in breast cancer: updates on clinical research and trials
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    Authors : Girithar et al., British Journal of Cancer
    2023-04
  • Tryptophan degradation enzymes expression in the placenta and the Kynurenine/Tryptophan ratio in maternal plasma after elective cesarean section
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    Authors : Silvano et al., Journal of Reproductive Immunology
    2023-02
  • Citrulline and kynurenine to tryptophan ratio: potential EED (environmental enteric dysfunction) biomarkers in acute watery diarrhea among children in Bangladesh
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    Authors : Das et al., Scientific Reports
    2023-01
  • Tryptophan degradation enzymes and Angiotensin (1−7) expression in human placenta
    Check the article
    Authors : Silvano et al., Journal of Reproductive Immunology
    2022-09
  • IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma
    Check the article
    Authors : Struckmeier et al., Journal of Cancer Reasearch & Clinical Oncology
    2022-08
  • Nociceptor-derived Reg3g prevents endotoxic death by targeting kynurenine pathway in microglia
    Check the article
    Authors : Sugisawa et al., Cell reports
    2022-03
  • Tryptophan Metabolism Is Associated with BMI and Adipose Tissue Mass and Linked to Metabolic Disease in Pediatric Obesity
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    Authors : Lischka et al, Nutrients
    2022-01
  • Apathy is not associated with a panel of biomarkers in older adults with HIV disease
    Check the article
    Authors : Wood et al., Journal of psychosomatic research
    2021-11
  • Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway
    Check the article
    Authors : Kim et al., Oncoimmunology
    2021-11
  • Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma
    Check the article
    Authors : Pospíšková et al., Pteridines
    2021-11
  • The Role of Indoleamine 2,3-Dioxygenase in Renal Tubular Epithelial Cells Senescence under Anoxia or Reoxygenation
    Check the article
    Authors : Eleftheriadis et al., Biomolecules
    2021-10
  • Plasma Kynurenine-to- Tryptophan Ratio, a Highly Sensitive Blood-Based Diagnostic Tool for Tuberculosis in Pregnant Women Living With Human Immunodeficiency Virus (HIV)
    Check the article
    Authors : Adu-Gyamfi et al., Clinical infectious Diseases
    2021-10
  • The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)
    Check the article
    Authors : Francikowski et al., Scientific reports
    2021-10
  • Abnormal Tryptophan Metabolism in HIV and Mycobacterium tuberculosis Infection
    Check the article
    Authors : Wang et al., Frontiers in microbiology
    2021-06
  • Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza
    Check the article
    Authors : Jung Cho et al., European Respiratory Journal
    2021-05
  • Role of indoleamine 2,3-dioxygenase in ischemia-reperfusion injury of renal tubular epithelial cells
    Check the article
    Authors : Eleftheriadis et al., Molecular Medicine Reports
    2021-04
  • Mood Disorder in Systemic Lupus Erythematosus Induced by Antiribosomal P Protein Antibodies Associated with Decreased Serum and Brain Tryptophan
    Check the article
    Authors : Cho et al., journal of Immunology
    2021-03
  • Early-Life Stress Modulates Gut Microbiota and Peripheral and Central Inflammation in a Sex-Dependent Manner
    Check the article
    Authors : Park et al., International journal of molecular sciences
    2021-02
  • Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans
    Check the article
    Authors : Baumgartner et al., Journal of internal medicine
    2020-08
  • Diagnostic accuracy of plasma kynurenine/tryptophan ratio, measured by enzyme-linked immunosorbent assay, for pulmonary tuberculosis
    Check the article
    Authors : Adu-Gyamfi et al., International journal of infectious diseases
    2020-08
  • Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients
    Check the article
    Authors : Masachika et al., Clinical neuroscience
    2020-06
  • Prognostic value of indoleamine 2,3 dioxygenase in patients with higher-risk myelodysplastic syndromes treated with azacytidine
    Check the article
    Authors : Müller-Thomas et al., British journal of haematology
    2020-04
  • Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8 + T-Cell-Dependent Tumor Activity
    Check the article
    Authors : Watanabe et al., Clinical Cancer Research
    2019-11
  • Blood Predictive Biomarkers for Patients With Non-small-cell Lung Cancer Associated With Clinical Response to Nivo_lumab
    Check the article
    Authors : Agullo-Ortuno et al., Clinical lung cancer
    2019-08
  • Traumatic injury leads to inflammation and altered tryptophan metabolism in the juvenile rabbit brain
    Check the article
    Authors : Zhang et al., Journal of Neurotrauma
    2018-09
  • Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
    Check the article
    Authors : Walker et al., Molecular Psychiatry
    2018-07
  • Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
    Check the article
    Authors : Martin-Hernandez et al., Molecular Neurobiology
    2018-05
  • Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
    Check the article
    Authors : Ziklo et al., Frontiers in cellular and infection microbiology
    2018-01
  • In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis
    Check the article
    Authors : Gautam et al., Proceedings of the National Academy of Sciences of the USA
    2018-01
  • Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
    Check the article
    Authors : Toulmonde et al., JAMA oncology
    2018-01
  • In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp
    Check the article
    Authors : Ziklo et al. BMC Microbiology
    2016-12

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