Metanephrine ELISA kit
High Sensitivity – Plasma samples

Ref: BA-E-8100
CatecholaminesDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
DatasheetMSDSProtocol

This Metanephrine ELISA kit enables the measurement of free L-metanephrine in plasma samples with high sensitivity. Intended for in vitro diagnostics & research use, this quantitative immunoassay is based on a simple and fast sample preparation protocol, and offers a limit of detection as low as 17pg/ml.

Sample type500µl plasma
Samples/kit40 samples in duplicate
Sensitivity17pg/ml
Range36 – 3 600pg/ml
Assay timeSample preparation and acetylation (30 min), ELISA overnight
ReactivityReacts with all species
CitationsCited in 4 papers
Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

476.00

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Quantity
Total

476.00

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Product overview

Product nameMetanephrine ELISA kit – Plasma
DescriptionIn vitro diagnostics & Research enzyme immunoassay (ELISA) for the sensitive determination of free metanephrine (metadrenaline) in plasma samples
LabelsIVD, CE
Format96-well plate
Samplesplasma
Sample volume500µL
ReactivityReacts with all species
Standard range0 / 36 – 3 600pg/ml
Sensitivity

17pg/ml

SpecificityNo significant cross-reactivity was observed with Metanephrine analogs such as Normetanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, VMS, HVMS, L-Dopa, L-Tyrosine, Tyramine and Acetaminophen
Assay timeSample preparation and acetylation 30 min and ELISA overnight
StorageStore at 2-8°C for to 6 months
DatasheetsInstructions for use, Material safety datasheet

 

Sample collection & storageEDTA- or citrate-plasma sample only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Sample preparationSample precipitation and acetylation (30min)
ELISAMetanephrine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolDownload instructions for use

Product citation

  • Comparison of plasma metanephrines measured by a commercial immunoassay and urinary catecholamines in the diagnosis of pheochromocytoma
    Check the article
    Authors : Christensen et al., Scandinavian journal of clinical and laboratory investigation
    2011-10
  • Evaluation of an enzyme immunoassay for plasma-free metanephrines in the diagnosis of catecholamine-secreting tumors
    Check the article
    Authors : Procopiou et al., European journal of Endocronology
    2009-04
  • Comparison of free plasma metanephrines enzyme immunoassay with (131)I-MIBG scan in diagnosis of pheochromocytoma
    Check the article
    Authors : Gao et al., Clinical and Experimental Medicine
    2008-07
  • Development of enantioselective immunoassays for free plasma metanephrines
    Check the article
    Authors : Manz et al., Annals of the New York Academy of Sciences
    2004-06

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