N8-Acetyl-Spermidine ELISA kit – Plasma, Serum, Supernatant

Ref: IS-I-2500R
Arginine metabolismResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

Polyamines are molecules derived from ornithine (Orn) (i.e., putrescine, spermine, spermidine) or arginine (Arg) (i.e., agmatine).  While ornithine decarboxylase is responsible for ornithine degradation and spermidine production, cSAT and nSAT transform spermidine to N8-acetylspermidine (N8-AcSpdn). These molecules are involved in many critical processes such as cell proliferation, nucleic acid synthesis, and cytoprotection from oxidative stress1. Several studies have evidenced that polyamine metabolism is frequently increased in cancer2 which can fuel tumor progression3 and favor immune escape4. Elevated levels of polyamines in sera of cancer patients have been demonstrated to correlate with poor prognosis.

Sample typePlasma, Cell culture supernatant
Capacity96 tests
Sensitivity0.5nM
Range2.4 – 93.75nM
Assay timeSample preparation 3h, ELISA overnight
ReactivityReacts with all species

 

Size

1 x 96 wells

539.00

99 in stock

Quantity
Total

539.00

Worldwide shipping

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Product overview

Product nameN8-Acetyl-Spermidine ELISA kit
DescriptionCompetitive ELISA kit for the quantitative measurement of N8-Acetyl-Spermidine (N8-AcSpdn) in plasma samples. For research use only
Format96-well plate
SamplesPlasma, Cell culture supernatant
Minimal sample volume20µL
ReactivityReacts with all species
Standard range2.4 – 93.75nM
Sensitivity

0.5nM

SpecificityNo significant cross-reactivity was observed with N1-Acetyl-Spermidine, Spermidine, Spermine, Ornithine & Putrescine
Assay timeSample preparation 3h and ELISA overnight
StorageStore at 2-8°C for up to 6 months
DatasheetsInstructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storageEDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparationSample preparation (3 hours)
ELISAN8-Acetyl-Spermidine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolDownload instructions for use

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