N8-Acetyl-Spermidine ELISA kit – Plasma, Serum, Supernatant

Ref: IS-I-2500R
Arginine metabolismResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

Polyamines are molecules derived from ornithine (Orn) (i.e., putrescine, spermine, spermidine) or arginine (Arg) (i.e., agmatine).  While ornithine decarboxylase is responsible for ornithine degradation and spermidine production, cSAT and nSAT transform spermidine to N8-acetylspermidine (N8-AcSpdn). These molecules are involved in many critical processes such as cell proliferation, nucleic acid synthesis, and cytoprotection from oxidative stress1. Several studies have evidenced that polyamine metabolism is frequently increased in cancer2 which can fuel tumor progression3 and favor immune escape4. Elevated levels of polyamines in sera of cancer patients have been demonstrated to correlate with poor prognosis.

Sample type Plasma, Cell culture supernatant
Capacity 96 tests
Sensitivity 0.5nM
Range 2.4 – 93.75nM
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species

 

Size

1 x 96 wells

539.00

99 in stock

Quantity
Total

539.00

Worldwide shipping

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Product overview

Product name N8-Acetyl-Spermidine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of N8-Acetyl-Spermidine (N8-AcSpdn) in plasma samples. For research use only
Format 96-well plate
Samples Plasma, Cell culture supernatant
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 2.4 – 93.75nM
Sensitivity

0.5nM

Specificity No significant cross-reactivity was observed with N1-Acetyl-Spermidine, Spermidine, Spermine, Ornithine & Putrescine
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storage EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA N8-Acetyl-Spermidine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

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