Serotonin ELISA kit
I Fast Assay I Serum and Urine

Ref: BA-E-8900
Tryptophan metabolismDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISANeuromediators
DatasheetMSDSProtocol

This Serotonin (5-HT) ELISA kit is tailored for the rapid measurement of 5-hydroxytryptamine in serum, urine and platelets. Intended for in vitro diagnostics, this fast-track quantitative immunoassay enables the determination of serotonin levels in up to 40 samples within 75 minutes.

Sample type 25µl serum, urine
Samples/kit 40 samples in duplicate
Sensitivity 8ng/ml
Range 8 – 2 170ng/ml
Assay time Sample acylation (15 min), ELISA (1h)
Reactivity Reacts with all species
Citations Cited in 4 papers

 

Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

353.00

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Quantity
Total

353.00

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Product overview

Product name Serotonin ELISA kit – Serum, Urine – Fast Track
Description In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of 5-hydroxytryptamine (5-HT, Serotonin) in serum or urine
Labels IVD, CE
Format 96-well plate
Samples 25 µl serum, urine
Reactivity Reacts with all species
Standard range 0 / 15 – 2 500ng/ml
Sensitivity

5ng/ml

Specificity No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan
Assay time 1h15
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for use, Material safety datasheet

 

Sample collection & storage Serum: Do not use haemolytic and especially lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C

Sample preparation Sample acylation (15min)
ELISA Serotonin antiserum incubation (30min), revelation and read steps (30min)
Detailed protocol Download instructions for use

Product citation

 

  • Dietary Supplementation with Lysine and Threonine Modulates the Performance and Plasma Metabolites of Broiler Chicken
    Check the article
    Authors : Ishii et al, The journal of poultry science
    2019-07
  • A novel murine model of severe pulmonary arterial hypertension
    Check the article
    Authors : Ciuclan et al, American journal of respiratory & clinical care medicine
    2011-08
  • Effects of delayed laboratory processing on platelet serotonin levels
    Check the article
    Authors : Sanner et al, Biological research for nursing
    2011-08
  • Relationship between chronic lead toxicity and plasma neurotransmitters in autistic patients from Saudi Arabia
    Check the article
    Authors : El-Ansary et al., Clinical biochemistry
    2011-07

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