L-Histidine ELISA kit – High Sensitivity – Plasma samples

Ref: IS-I-1300R
HistamineAmino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

L-Histidine (His) is an essential amino acid displaying a unique properties profile, involved in a number of biological activities, e.g. in pH buffering, in metal ion chelation, and in oxidative species scavenging, which confer it many physiological properties as antioxidant, anti-inflammatory, and in erythropoiesis and immune system regulation.

While there are several pathways of His degradation, most catabolism is directed to protein turnover and glutamate production and begins with histidase-dependent deamination. Also, the minor pathways of His as a precursor of histamine and His-rich proteins and peptides make it unique among other amino acids.

Sample typePlasma
Capacity96 tests
Sensitivity1.4µM
Range10 – 390µM
Assay timeSample preparation 3h, ELISA overnight
ReactivityReacts with all species

 

Size

1 x 96 wells

539.00

89 in stock

Quantity
Total

539.00

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Product overview

Product nameL-Histidine ELISA kit
DescriptionCompetitive ELISA kit for the quantitative measurement of L-Histidine (His) in plasma samples. For research use only
Format96-well plate
SamplesPlasma
Minimal sample volume10µL
ReactivityReacts with all species
Standard range5.3 – 390µM (1.1 – 81.8µg/mL)
Sensitivity

1.4µM

SpecificityNo significant cross-reactivity was observed with Histamine dihydrochloride, L-Glutamic acid, 3-Methyl-L-histidine & L-Histidinol dihydrochloride
Assay timeSample preparation 3h and ELISA overnight
StorageStore at 2-8°C for up to 6 months
DatasheetsInstructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storageEDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparationSample preparation (3 hours)
ELISAL-Histidine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolDownload instructions for use

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