Imaging neurotransmitters, not enzymes, receptors, or transporters
April 10, 2024 2025-06-05 15:45Imaging neurotransmitters, not enzymes, receptors, or transporters
Imaging neurotransmitters, not enzymes, receptors, or transporters
Dopamine, Serotonin, GABA, Glutamate, Histamine, Norepinephrine, Serine, Octopamine, … To generate spatial information about key neuromodulators and neurotransmitters, neurobiologists typically perform stainings against enzymes (e.g. TH for dopamine, GAD65-67 for GABA), receptors (e.g. Glutamate) or transporters (e.g. Norepinephrine, Glutamate). Why? Simply because highly specific antibodies against neuromediators weren’t available until recently.
Our team developed and IF/IHC-validated 17 primary antibodies to key neurotransmitters. These antibodies were already used in 20+ papers. Take a look below. And for easy quantification, see our ELISA kits to neurotransmitters.
Staining for neurotransmitters: a few pictures
GABA (green) & MAP2 (red) in adult mouse primary cortical neurons
Immunodetection of MAP2 (red) and GABA (green) mouse primary cortical cultures, using anti-GABA IS1006 antibody and the STAINperfect immunostaining kit.
L-Glutamate (green) & MAP2 (red) in mouse cortical primary neurons
Immunodetection of L-Glutamate (green) and MAP2- (red) positive neurons in mouse primary cortical culture reveals the presence of L-Glutamate within fibers and soma of neurons (IS1001 anti-glutamate rabbit pAb)
Octopamine (green) & Serotonin (red) in crayfish eyestalk
Immunostaining of crayfish eyestalk using anti-octopamine rabbit polyclonal antibody (green) and anti-serotonin goat polyclonal antibody (red). Tissues were processed with whole mount protocol of STAINperfect kit A.
Serotonin in FFPE human colon tissue section (IHC)
Rabbit polyclonal antibody IS1086Â highlights the presence of 5-HT enterochromaffin cells of the intestinal mucosa.
Give it a try!
Our team developed 17 antibodies to key neurotransmitters, together with a dedicated immunostaining kit for straightforward multiplexed IF/IHC imaging in cell cultures, whole mounts, and tissue sections.
A trial pack is available, containing the STAINperfect staining kit and 2 antibodies.
If you have any questions about staining protocols or sample preparation, ask us ! Our antibodies were used in 20+ papers to stain stem cell-derived midbrain organoids, spider tissues, human brain tissues, and more. So we might have answers for you!
And to quantify neurotransmitters in supernatant and biological samples, we developed highly sensitive ELISA kits, cross-validated by LC-MS, which have already been cited in 100+ papers.