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D-Serine Antibody – Rabbit Polyclonal
Ref: IS1004NeuromediatorsAmino acidsPolyclonal AntibodiesICCIFIHC
+D-Serine imaging in adult mouse frontal cortex
Using STAINperfect immunostaining kit A and following protocol developped for tissu sections, the anti-D-Serine rabbit polyclonal antibody (Ref: IS1004) revealed the presence of D-Serine postive cells within mouse frontal cortex with a cytoplasmic pattern. The staining was revealed using Alexa Fluor® 488-conjugated secondary antibody and image obtained using confocal imaging.
DatasheetMSDS
The anti-D-Serine antibody IS1004 was validated for immunofluorescence in mouse brain tissues, in combination with the STAINperfect immunostaining kit A. Following the same staining procedure, this rabbit polyclonal antibody can be used to visualize D-Serine in whole mounts, cell cultures, and tissue sections (IF or IHC).
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Eligible samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
Product overview
| Product name | D-Serine antibody – Rabbit pAb |
| Synonyms | Anti-D-Serine antibody Anti (R)-Serine antibody |
| Immunogen | Conjugated D-Serine |
| Specificity | When tested in competitive ELISA, the anti-conjugated D-Serine antibody did not show any significant cross reactivity with D-Serine analogs, including L-Serine and D-Threonine |
| Lot number | 140301 |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for D-Serine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
Selected publications on D-Serine:
- Paul P, de Belleroche J. The role of D-serine and glycine as co-agonists of NMDA receptors in motor neuron degeneration and amyotrophic lateral sclerosis (ALS). Front Synaptic Neurosci. 2014 Apr 16;6:10.
- Balu DT et al. D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain. Cell Mol Neurobiol. 2014 Apr;34(3):419-35. doi: 10.1007/s10571-014-0027-z. Epub 2014 Jan 17.
- Klatte K et al. Impaired D-serine-mediated cotransmission mediates cognitive dysfunction in epilepsy. J Neurosci. 2013 Aug 7;33(32):13066-80.
- Otte DM et al. Effects of Chronic D-Serine Elevation on Animal Models of Depression and Anxiety-Related Behavior. PLoS One. 2013 Jun 21;8(6):e67131.
- Martineau M et al. Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci. 2013 Feb 20;33(8):3413-23. doi: 10.1523/JNEUROSCI.3497-12.2013.
- Van Horn MR, Sild M, Ruthazer ES. D-serine as a gliotransmitter and its roles in brain development and disease. Front Cell Neurosci. 2013 Apr 23;7:39. doi: 10.3389/fncel.2013.00039.
