Antibodies & immunoassays for small molecules Kynurenine/Tryptophan ratio ELISA pack
Ref: ISE-2227
Kynurenine pathwayTryptophan metabolismDiagnostic ELISA kits (IVD)ELISA
+Kynurenine-to-Tryptophan ratio by ELISA
The kynurenine-tryptophan ratio (KTR) is a key marker of tryptophan catabolism along the kynurenine pathway, through the enzymatic activity of indoleamine 2,3-dioxygenase (IDO1/2) or tryptophan 2,3-dioxygenase (TDO2). KTR has been described to be increased in a wide range of pathological contexts, including cancers, infectious diseases, as well as neurological disorders.
DatasheetMSDS TryptophanProtocol TryptophanMSDS KynurenineProtocol Kynurenine
The Kynurenine/Tryptophan ratio ELISA pack enables quantitative measurements of tryptophan catabolism along the kynurenine pathway in large numbers of samples. The two kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 20µl, they permit the performance of longitudinal studies in mice.
| Sample type | Plasma, Serum, Cell culture supernatant, (Urine) |
| Kit capacity | 2 x 96 tests |
| Sensitivity | LoD KYN: < 47.5ng/ml LoD TRP: < 1.2µg/ml |
| Range | KYN: 0/100 – 10000ng/ml TRP: 2.5 – 250µg/ml |
| Reactivity | Reacts with all species |
| References | Citations in literature |
Product overview
| Product name | Kynurenine/Tryptophan ratio ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine to L-Tryptophan ratio in urine, plasma and serum samples |
| Labels | CE |
| Format | 2 x 96-well plate |
| Samples | Plasma, serum, cell culture supernatant, (Urine) |
| Minimum sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | KYN: 0/100 – 10000ng/ml TRP: 2.5 – 250µg/ml |
| Sensitivity |
LoD KYN: < 47.5ng/ml |
| Specificity | No significant cross-reactivity was observed with L-Tryptophan and L-Kynurenine analogs. See product pages for L-Kynurenine ELISA and L-Tryptophan ELISA RUO |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use – TRP kit, Instructions for use -KYN kit |
| Packs intended for in-vitro diagnostic (IVD) are not available for the US-market.
In contrast to packs for in-vitro-diagnostic (IVD), packs meant for research use only (RUO) do have a R in the last position of the catalogue number. |
| Sample collection & storage | Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | KYN: Sample acylation (90 min) TRP: Sample precipitation (15 min) and derivatization (2h) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Instructions for use – TRP kit, Instructions for use – KYN kit |
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