Antibodies & immunoassays for small molecules Kynurenine/Tryptophan ratio ELISA pack
Ref: ISE-2227
Kynurenine pathwayTryptophan metabolismDiagnostic ELISA kits (IVD)ELISA
+Kynurenine-to-Tryptophan ratio by ELISA
The kynurenine-tryptophan ratio (KTR) is a key marker of tryptophan catabolism along the kynurenine pathway, through the enzymatic activity of indoleamine 2,3-dioxygenase (IDO1/2) or tryptophan 2,3-dioxygenase (TDO2). KTR has been described to be increased in a wide range of pathological contexts, including cancers, infectious diseases, as well as neurological disorders.
DatasheetMSDS TryptophanProtocol TryptophanMSDS KynurenineProtocol Kynurenine
The Kynurenine/Tryptophan ratio ELISA pack enables quantitative measurements of tryptophan catabolism along the kynurenine pathway in large numbers of samples. The two kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 20µl, they permit the performance of longitudinal studies in mice.
| Sample type | Plasma, Serum, Cell culture supernatant, (Urine) |
| Kit capacity | 2 x 96 tests |
| Sensitivity | LoD KYN: < 47.5ng/ml LoD TRP: < 1.2µg/ml |
| Range | KYN: 0/100 – 10000ng/ml TRP: 2.5 – 250µg/ml |
| Reactivity | Reacts with all species |
| References | Citations in literature |
Product overview
| Product name | Kynurenine/Tryptophan ratio ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine to L-Tryptophan ratio in urine, plasma and serum samples |
| Labels | CE |
| Format | 2 x 96-well plate |
| Samples | Plasma, serum, cell culture supernatant, (Urine) |
| Minimum sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | KYN: 0/100 – 10000ng/ml TRP: 2.5 – 250µg/ml |
| Sensitivity |
LoD KYN: < 47.5ng/ml |
| Specificity | No significant cross-reactivity was observed with L-Tryptophan and L-Kynurenine analogs. See product pages for L-Kynurenine ELISA and L-Tryptophan ELISA RUO |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use – TRP kit, Instructions for use -KYN kit |
| Packs intended for in-vitro diagnostic (IVD) are not available for the US-market.
In contrast to packs for in-vitro-diagnostic (IVD), packs meant for research use only (RUO) do have a R in the last position of the catalogue number. |
| Sample collection & storage | Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | KYN: Sample acylation (90 min) TRP: Sample precipitation (15 min) and derivatization (2h) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Instructions for use – TRP kit, Instructions for use – KYN kit |
Product citation
- Tryptophan degradation enzymes and Angiotensin (1−7) expression in human placenta
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Authors : Silvano et al., Journal of Reproductive Immunology
2022-09 - Nociceptor-derived Reg3g prevents endotoxic death by targeting kynurenine pathway in microglia
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Authors : Sugisawa et al., Cell reports
2022-03 - Tryptophan Metabolism Is Associated with BMI and Adipose Tissue Mass and Linked to Metabolic Disease in Pediatric Obesity
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Authors : Lischka et al, Nutrients
2022-01 - The Role of Indoleamine 2,3-Dioxygenase in Renal Tubular Epithelial Cells Senescence under Anoxia or Reoxygenation
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Authors : Eleftheriadis et al., Biomolecules
2021-10 - The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)
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Authors : Francikowski et al., Scientific reports
2021-10 - Plasma Kynurenine-to- Tryptophan Ratio, a Highly Sensitive Blood-Based Diagnostic Tool for Tuberculosis in Pregnant Women Living With Human Immunodeficiency Virus (HIV)
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Authors : Adu-Gyamfi et al., Clinical infectious Diseases
2021-10 - Abnormal Tryptophan Metabolism in HIV and Mycobacterium tuberculosis Infection
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Authors : Wang et al., Frontiers in microbiology
2021-06 - Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza
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Authors : Jung Cho et al., European Respiratory Journal
2021-05 - Paliperidone Reversion of Maternal Immune Activation-Induced Changes on Brain Serotonin and Kynurenine Pathways
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Authors : MacDowell et al., Frontiers in pharmacology
2021-05 - Role of indoleamine 2,3-dioxygenase in ischemia-reperfusion injury of renal tubular epithelial cells
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Authors : Eleftheriadis et al., Molecular Medicine Reports
2021-04 - Serum kynurenine levels are a novel biomarker to predict the prognosis of patients with hepatocellular carcinoma
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Authors : Bekki et al., PloS one
2020-10 - Diagnostic accuracy of plasma kynurenine/tryptophan ratio, measured by enzyme-linked immunosorbent assay, for pulmonary tuberculosis
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Authors : Adu-Gyamfi et al., International journal of infectious diseases
2020-08 - Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans
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Authors : Baumgartner et al., Journal of internal medicine
2020-08 - Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients
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Authors : Masachika et al., Clinical neuroscience
2020-06 - Prognostic value of indoleamine 2,3 dioxygenase in patients with higher-risk myelodysplastic syndromes treated with azacytidine
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Authors : Müller-Thomas et al., British journal of haematology
2020-04 - IDO Targeting in Sarcoma: Biological and Clinical Implications
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Authors : Nafia et al., Frontiers in immunology
2020-03 - Blood Predictive Biomarkers for Patients With Non-small-cell Lung Cancer Associated With Clinical Response to Nivolumab
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Authors : Agullo-Ortuno et al., Clinical lung cancer
2019-08 - Traumatic injury leads to inflammation and altered tryptophan metabolism in the juvenile rabbit brain
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Authors : Zhang et al., Journal of Neurotrauma
2018-09 - Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
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Authors : Walker et al., Molecular Psychiatry
2018-07 - Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
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Authors : Martin-Hernandez et al., Molecular Neurobiology
2018-05 - Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
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Authors : Toulmonde et al., JAMA oncology
2018-01 - Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
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Authors : Ziklo et al., Frontiers in cellular and infection microbiology
2018-01
