Antibodies & immunoassays for small molecules L-Serine ELISA kit
Ref: IS-I-1200R
+# IS-I-1200 : L-Serine (Ser) ELISA kit
Validated IS-I-1200 ELISA kit allows for the determination of L-Serine (Ser) in plasma samples of a volume as low as 10μL and with a sensitivity of 2.4µM.
L-Serine (L-Ser) is a non-essential amino acid which becomes conditionally essential – in certain circumstances – particularly when it cannot be synthesized in a sufficient amount to meet the cellular demands. L-Ser may be derived from different sources including dietary intake, the glycolytic intermediate 3-phosphoglycerate, glycine as well as protein and phospholipid degradation. It’s worth noting that enzymes involved in L-Ser biosynthesis are upregulated in highly proliferating cells, including cancer cells, to limit L-Ser over-consumption. Besides its essential role in cell proliferation, L-Ser is important for brain development and functions which are especially sustained by i) altered L-Ser levels in psychiatric disorders and ii) occurrence of severe neurological abnormalities in patients with defects in L-Ser biosynthesis.
| Sample type | Plasma, Serum, Cell culture supernatant |
| Capacity | 96 tests |
| Sensitivity | 2.4µM |
| Range | 3.8 – 625µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | L-Serine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of L-Serine (Ser) in plasma and serum samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma, Serum, Cell supernatant |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 3.8 – 625µM |
| Sensitivity |
2.4µM |
| Specificity | No significant cross-reactivity was observed with Glycine, L-Threonine, L-Cysteine and D-Serine |
| Assay time | Sample preparation 3h and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
For research use only – Do not use for diagnostic
| Sample collection & storage | EDTA Plasma
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Serine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Selected articles on Serine:
- Mattaini et al., The importance of serine metabolism in cancer, J Cell Biol, 2016
- Tabatabaie et al., L-serine synthesis in the central nervous system: a review on serine deficiency disorders, Mol Genet Metab, 2010
- Koning et al., L-serine in disease and development, Biochemical Journal, 2003
