Antibodies & immunoassays for small molecules L-Serine ELISA kit
Ref: IS-I-1200R
+Typical standard curve of Serine ELISA
Typical standard curve obtained with the Ser ELISA kit where the optical density is inversely correlated within levels within a linear range of 3.8 - 625μM. (example data - do not use for calculation)
L-Serine (L-Ser) is a non-essential amino acid which becomes conditionally essential – in certain circumstances – particularly when it cannot be synthesized in a sufficient amount to meet the cellular demands. L-Ser is important for brain development and functions which are especially sustained by altered L-Ser levels in psychiatric disorders and occurrence of severe neurological abnormalities in patients with defects in L-Ser biosynthesis.
The L-Ser ELISA kit allows for the determination of Ser in cell culture supernatant, serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies
| Sample type | Plasma, Serum, Cell culture supernatant |
| Capacity | 96 tests |
| Sensitivity | 2.4µM |
| Range | 3.8 – 625µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | L-Serine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of L-Serine (Ser) in plasma and serum samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma, Serum, Cell supernatant |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 3.8 – 625µM |
| Sensitivity |
2.4µM |
| Specificity | No significant cross-reactivity was observed with Glycine, L-Threonine, L-Cysteine and D-Serine |
| Assay time | Sample preparation 3h and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
For research use only – Do not use for diagnostic
| Sample collection & storage | EDTA Plasma
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Serine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Selected articles on Serine:
- Mattaini et al., The importance of serine metabolism in cancer, J Cell Biol, 2016
- Tabatabaie et al., L-serine synthesis in the central nervous system: a review on serine deficiency disorders, Mol Genet Metab, 2010
- Koning et al., L-serine in disease and development, Biochemical Journal, 2003
