L-Lysine ELISA kit

Ref: IS-I-1400R
Amino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

L-Lysine (Lys) is an essential amino acid since it’s not produced in the body and must be supplied through the diet. It’s one of the building blocks for protein, peptide and non-peptide synthesis, and is thus found involved in several physiological and biochemical processes. As a result, Lys deficiency was shown to affect cell cycle, apoptosis, amino acid metabolism, and to even impair antibody responses as well as cell-mediated immune cell functions. In addition, and like other amino acids, Lys contributes to epigenetic and protein regulations and immunosuppression ; its catabolism activation generates acetyl-CoA which, for instance, promotes Wnt signaling and can support tumor growth.

Furthermore, imbalance in circulating Lys levels can be observed in many conditions such as i) inherited diseases on enzymes involved in Lys catabolism (e.g. glutaric aciduria type I or antiquitin deficiency), ii) diabetes-dependent cardiovascular disease, as well as iii) human immunodeficiency viruses-1 infection.

Sample type Plasma
Capacity 96 tests
Sensitivity 13µM  (2.4µg/mL)
Range 16 – 650µM  (3 – 118µg/mL)
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species

 

Size

1 x 96 wells

490.00

100 in stock

Quantity
Total

490.00

Worldwide shipping

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Product overview

Product name L-Lysine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Lysine (Lys) in plasma samples. For research use only
Format 96-well plate
Samples Plasma
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 16 – 650µM
Sensitivity

13µM

Specificity No significant cross-reactivity was observed with Cadaverine dihydrochloride, L-HomoArginine hydrochloride, N-methyl-L-Lysine hydrichloride and N-acetyk-L-Lysine
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storage EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA L-Lysine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

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