Staining of L-Serine and GABA positive neurons in mouse primary cortical culture
Primary mouse cortical neurons were stained using anti-L-Serine (green) polyclonal rabbit antibody (IS1003) and anti-GABA (red)chicken polyclonal antibody (IS1036) using the STAINperfect immunostaining kit A and according to the protocol for cell culture. Fluorescent conjugated secondary antibodies were used and picture obtained by confocal imaging. Co-immunostaining appears in yellow.
L-Serine antibody - Rabbit polyclonal AbRef: IS1003
The rabbit polyclonal antibody directed against conjugated L-Serine IS1003 is highly affine and specific. In combination with the STAINperfect immunostaining kit A, the antibody directly labels L-serine in whole mounts, cell culture and tissue sections.
|Species reactivity||Reacts with all species
|Tested samples||whole mounts, cell culture, tissue sections|
|Staining procedure||STAINperfect immunostaining kit A|
|Format||50µL (approx. 40 tissue sections)|
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
L-Serine antibody - Rabbit pAb
Anti-L-Serine acid antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated L-Serine antibody did not show any significant cross reactivity with L-Serine analogs, including D-Serine and L-Threonine conjugates|
Store at +4°C for short term (1-2 monts). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
|IF - Cell cultures, Whole mounts, Tissue sections||
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for L-Serine staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
Antibody not yet cited. Submit an article and get a 10% discount.
Selected publications about L-Serine:
- Pollari S et al. Enhanced serine production by bone metastatic breast cancer cells stimulates osteoclastogenesis. Breast Cancer Res Treat. 2011 Jan;125(2):421-30. doi: 10.1007/s10549-010-0848-5. Epub 2010 Mar 30.
- Yang JH et al. Brain-specific Phgdh deletion reveals a pivotal role for L-serine biosynthesis in controlling the level of D-serine, an N-methyl-D-aspartate receptor co-agonist, in adult brain. J Biol Chem. 2010 Dec 31;285(53):41380-90. doi: 10.1074/jbc.M110.187443. Epub 2010 Oct 21.
- Tabatabaie L, Klomp LW, Berger R, de Koning TJ. L-serine synthesis in the central nervous system: a review on serine deficiency disorders. Mol Genet Metab. 2010 Mar;99(3):256-62. doi: 10.1016/j.ymgme.2009.10.012. Epub 2009 Oct 25.