Staining of L-Serine and GABA positive neurons in mouse primary cortical culture
Primary mouse cortical neurons were stained using anti-L-Serine (green) polyclonal rabbit antibody (IS1003) and anti-GABA (red)chicken polyclonal antibody (IS1036) using the STAINperfect immunostaining kit A and according to the protocol for cell culture. Fluorescent conjugated secondary antibodies were used and picture obtained by confocal imaging. Co-immunostaining appears in yellow.
L-Serine antibody - Rabbit polyclonal Ab
Ref: IS1003
The rabbit polyclonal antibody directed against conjugated L-Serine IS1003 is highly affine and specific. In combination with the STAINperfect immunostaining kit A, the antibody directly labels L-serine in whole mounts, cell culture and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Species reactivity | Reacts with all species |
| Tested samples | whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name |
L-Serine antibody - Rabbit pAb |
| Synonyms |
Anti-L-Serine acid antibody |
| Immunogen | Conjugated L-Serine |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Serine antibody did not show any significant cross reactivity with L-Serine analogs, including D-Serine and L-Threonine conjugates |
| Lot number |
140301 |
Storage
| Form |
Liquid |
| Purity | Purified anti-serum |
| Storage buffer |
Store at +4°C for short term (1-2 monts). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| IF - Cell cultures, Whole mounts, Tissue sections |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for L-Serine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
References
Antibody not yet cited. Submit an article and get a 10% discount.
Selected publications about L-Serine:
- Yang JH et al. Brain-specific Phgdh deletion reveals a pivotal role for L-serine biosynthesis in controlling the level of D-serine, an N-methyl-D-aspartate receptor co-agonist, in adult brain. J Biol Chem. 2010 Dec 31;285(53):41380-90. doi: 10.1074/jbc.M110.187443. Epub 2010 Oct 21.
- Tabatabaie L, Klomp LW, Berger R, de Koning TJ. L-serine synthesis in the central nervous system: a review on serine deficiency disorders. Mol Genet Metab. 2010 Mar;99(3):256-62. doi: 10.1016/j.ymgme.2009.10.012. Epub 2009 Oct 25.
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