Antibodies & immunoassays for small molecules Dopamine ELISA kit – Research
Plasma & Urine samples
Ref: BA-E-6300R
NeuromediatorsCatecholaminesResearch ELISA kits (RUO)ELISA
+Dopamine ELISA kit
DatasheetMSDSProtocol
This Dopamine ELISA kit is tailored for the rapid measurement of Dopamine (DA) in urine and plasma samples. Intended for research use only, this fast-track quantitative immunoassay enables to determine dopamine levels in up to 40 samples within 4 hours.
| Sample type | 10µl urine, 300µl plasma |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 4.5ng/ml urine, 25pg/ml plasma |
| Range | 4.5 – 2 000ng/ml |
| Assay time | Sample extraction and acylation (65 min), ELISA (3h) |
| Reactivity | Reacts with all species |
| Citations | 1 |
Product overview
| Product name | Dopamine ELISA kit – Urine/Plasma – Fast-Track |
| Description | Competitive ELISA kit for the quantitative measurement of Dopamine levels in plasma and urine samples. For research use only |
| Labels | RUO |
| Format | 96-well plate |
| Samples | 10µl urine, 300µl plasma |
| Reactivity | Reacts with all species |
| Standard range | 0 / 4.5 – 2 000ng/ml |
| Sensitivity |
4.5ng/ml urine, 25pg/ml plasma |
| Specificity | No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid |
| Assay time | 4 hours |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalog no. BA E-6300. |
| Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C |
| Sample preparation | Sample preparation, extraction and acylation (65min) |
| ELISA | Dopamine antiserum incubation (2H), revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy
Check the article
Authors : Gaber et al., The British journal of Diabetes & Vascular Disease
2013-05
