Dopamine ELISA kit – Research
Plasma & Urine samples

Ref: BA-E-6300R
NeuromediatorsResearch ELISA kits (RUO)ELISA
DatasheetMSDSProtocol

This Dopamine ELISA kit is tailored for the rapid measurement of Dopamine (DA) in urine and plasma samples. Intended for research use only, this fast-track quantitative immunoassay enables to determine dopamine levels in up to 40 samples within 4 hours.

Sample type 10µl urine, 300µl plasma
Samples/kit 40 samples in duplicate
Sensitivity 4.5ng/ml urine, 25pg/ml plasma
Range 0 / 4.5 – 2 000ng/ml
Assay time Sample extraction and acylation (65 min), ELISA (3h)
Reactivity Reacts with all species
Citations 1
Size

1 x 96 wells

410.00

100 in stock

Quantity
Total

410.00

Worldwide shipping

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Product overview

Product name Dopamine ELISA kit – Urine/Plasma – Fast-Track
Description Competitive ELISA kit for the quantitative measurement of Dopamine levels in plasma and urine samples. For research use only
Labels RUO
Format 96-well plate
Samples 10µl urine, 300µl plasma
Reactivity Reacts with all species
Standard range 0 / 4.5 – 2 000ng/ml
Sensitivity

4.5ng/ml urine, 25pg/ml plasma

Specificity No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid
Assay time 4 hours
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for use, Material safety datasheet

 

The kit for in-vitro-diagnostic (IVD) use  can be ordered with the catalogue no. BA E-6300.

Sample collection & storage Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C

Sample preparation Sample preparation, extraction and acylation (65min)
ELISA Dopamine antiserum incubation (2H), revelation and read steps (1h)
Detailed protocol Download instructions for use

Product citation

  • The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy
    Check the article
    Authors : Gaber et al., The British journal of Diabetes & Vascular Disease
    2013-05

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A key neurotransmitter in the brain, Dopamine (DA) does not cross the blood-brain barrier. Found at concentrations similar to those of epinephrine in plasma, free dopamine is mainly produced at the peripheral level by the sympathetic nervous system and the digestive system. In humans, at least 95% of plasma dopamine circulates in the form of dopamine sulfate, a conjugate produced by mesentric organs. Playing a key role as a regulator of blood flow, circulating dopamine also exerts exocrine and paracrine effects on immune cells, the pancreas and the kidney.

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