Antibodies & immunoassays for small molecules Dopamine ELISA kit – Research
Plasma & Urine samples
Ref: BA-E-6300R
NeuromediatorsResearch ELISA kits (RUO)ELISA
+Research ELISA kit for Dopamine quantification
DatasheetMSDSProtocol
This Dopamine ELISA kit is tailored for the rapid measurement of Dopamine (DA) in urine and plasma samples. Intended for research use only, this fast-track quantitative immunoassay enables to determine dopamine levels in up to 40 samples within 4 hours.
| Sample type | 10µl urine, 300µl plasma |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 4.5ng/ml urine, 25pg/ml plasma |
| Range | 0 / 4.5 – 2 000ng/ml |
| Assay time | Sample extraction and acylation (65 min), ELISA (3h) |
| Reactivity | Reacts with all species |
| Citations | 1 |
Product overview
| Product name | Dopamine ELISA kit – Urine/Plasma – Fast-Track |
| Description | Competitive ELISA kit for the quantitative measurement of Dopamine levels in plasma and urine samples. For research use only |
| Labels | RUO |
| Format | 96-well plate |
| Samples | 10µl urine, 300µl plasma |
| Reactivity | Reacts with all species |
| Standard range | 0 / 4.5 – 2 000ng/ml |
| Sensitivity |
4.5ng/ml urine, 25pg/ml plasma |
| Specificity | No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid |
| Assay time | 4 hours |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalogue no. BA E-6300. |
| Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C |
| Sample preparation | Sample preparation, extraction and acylation (65min) |
| ELISA | Dopamine antiserum incubation (2H), revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy
Check the article
Authors : Gaber et al., The British journal of Diabetes & Vascular Disease
2013-05
