D-Glutamate rabbit pAb – IS1002Ref: IS1002-sp
This anti-D-Glutamate rabbit polyclonal antibody IS1002 was developped to ensure superior affinity and specificity. Combined with the STAINperfect immunostaining kit A, this antibody allows direct D-Glutamic acid visualization in cell cultures, whole mounts and tissue sections.
|Reactivity||Reacts with all species|
|Eligible samples||Whole mounts, cell culture, tissue sections|
|Staining procedure||STAINperfect immunostaining kit A|
|Format||50µL (approx. 40 tissue sections)|
|Product name||D-Glutamate antibody – Rabbit pAb|
|Synonyms||Anti-D-Glutamic acid antibody|
|Specificity||When tested in competitive ELISA, the anti-conjugated D-Glutamate antibody did not show any significant cross reactivity with D-Glutamate analogs, L-Glutamate and D-Aspartate|
Reconstitution & storage
|Storage buffer||Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles|
|Material safety datasheet||Download MSDS|
|IF – Cell cultures, Whole mounts, Tissue sections||Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample|
|Comments||Optimal working dilutions must be determined by the end-user|
|Restrictions||For research use only|
|Full protocol||Download STAINperfect protocol for D-Glutamate staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
Antibody not yet cited. Submit an article and get a 10% discount.
Selected papers on D-Glutamic acid:
- Dayer MR and Dayer MS. Hypoglycemic Effects of D-glutamic Acid in Diabetic Rats International Journal of Pharmacology, 2010 Nov 6: 930-936.
- Mangas A et al. Immunocytochemical visualization of D-glutamate in the rat brain. Neuroscience. 2007 Jan 19;144(2):654-64. Epub 2006 Nov 3.
- Fisher GH et al. Presence of D-aspartate and D-glutamate in tumor proteins. Cancer Biochem Biophys. 1995 Nov;15(2):79-82.
- Kera Y et al. Presence of free D-glutamate and D-aspartate in rat tissues. Biochim Biophys Acta. 1995 Feb 23;1243(2):283-6.