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STAINperfect™ – Trial pack
Ref: SP-T-0001IFStaining kits
+GABAergic network and glutamatergic cell populations
in a mature rat cortical culture revealed by GABA and L-glutamate immunoreactivity detection using mouse monoclonal (#IS039) and rabbit polyclonal (#IS1001) antibodies, respectively, with the STAINperfect immunostaining kit A.
DatasheetMSDSProtocol
The trial pack STAINperfect™ allows testing the STAINperfect™ immunostaining kit A in combination with 2 primary antibodies to key neurotransmitters and small molecules. The kit is provided with all necessary reagents and protocols to test the STAINperfect™ procedure in cell cultures, whole mounts and brain tissue sections.
| Contents | 1x STAINperfect™ immunostaining kit A 2x 25µl antibody vials |
| Number of stainings | Cell cultures : 1x 96 well-plate Whole mounts : 20 samples Tissue sections: 40 slides |
| References | Cited in 3 papers |
Product overview
| Product name | STAINperfect™ Neurotransmiters – Immunostaining Trial Pack |
| Description | Trial offer consisting of a STAINperfect™ immunostaining kit A and 2 primary antibodies in 25µl format. |
| Eligible samples | Cell cultures, whole mounts, tissue sections (any species) |
| Number of stainings | Cell cultures : 1x 96 well-plate Whole mounts : 20 samples Tissue sections: 40 slides |
| Storage | Store at 4C° until use |
| Restriction | For research use only |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibodies with the antibody diluent provided in the STAINperfect™ immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect™ protocol suited to your sample. |
| Comments | Optimal working dilutions must be determined by the end-user. Third-party anti-rabbit secondary antibody required. |
| Protocols |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
Product citations
- Gad1 knock-out rats exhibit abundant spike-wave discharges in EEG, exacerbated with valproate treatment
Check the article
Authors : Liu et al., Frontiers in Immunology
2023-09 - Monocarboxylate Transporter-1 (MCT1)-Mediated Lactate Uptake Protects Pancreatic Adenocarcinoma Cells from Oxidative Stress during Glutamine Scarcity Thereby Promoting Resistance against Inhibitors of Glutamine Metabolism
Check the article
Authors : Ammar et al., Antioxydants
2023-09 - Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
Check the article
Authors : Smits et al., Cell & tissue research
2020-07
