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L-Histidine ELISA kit – High Sensitivity – Plasma samples
Ref: IS-I-1300RHistamineAmino acidsResearch ELISA kits (RUO)ELISA
+L-Histidine ELISA kit
DatasheetProtocolMSDS
L-Histidine (His) is an essential amino acid displaying a unique properties profile, involved in a number of biological activities, e.g. in pH buffering, in metal ion chelation, and in oxidative species scavenging, which confer it many physiological properties as antioxidant, anti-inflammatory, and in erythropoiesis and immune system regulation.
While there are several pathways of His degradation, most catabolism is directed to protein turnover and glutamate production and begins with histidase-dependent deamination. Also, the minor pathways of His as a precursor of histamine and His-rich proteins and peptides make it unique among other amino acids.
| Sample type | Plasma |
| Capacity | 96 tests |
| Sensitivity | 1.4µM |
| Range | 10 – 390µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
Product overview
| Product name | L-Histidine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of L-Histidine (His) in plasma samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma |
| Minimal sample volume | 10µL |
| Reactivity | Reacts with all species |
| Standard range | 5.3 – 390µM (1.1 – 81.8µg/mL) |
| Sensitivity | 1.4µM |
| Specificity | No significant cross-reactivity was observed with Histamine dihydrochloride, L-Glutamic acid, 3-Methyl-L-histidine & L-Histidinol dihydrochloride |
| Assay time | Sample preparation 3h and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
For research use only – Do not use for diagnostic
| Sample collection & storage | EDTA Plasma Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Histidine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Selected articles on Histidine
- Holecek et al., Histidine in Health and Disease: Metabolism, Physiological Importance, and Use as a Supplement, Nutrients, 2020
- Poon et al., Histidine-rich glycoprotein: the Swiss Army knife of mammalian plasma, Blood, 2010
- Li et al., Amino acids and immune function, Br J Nutr, 2007
- Tsukano et al., Plasma histidine concentrations as a specific biomarker for intestinal mucosal damage in calves with cryptosporidiosis, Res Vet Sci, 2020
- Kanarek et al., Histidine catabolism is a major determinant of methotrexate sensitivity, Nature, 2018
- Frezza et al., Histidine metabolism boosts cancer therapy, Nature, 2018
- Petrova et al., Potential Benefits and Pitfalls of Histidine Supplementation for Cancer Therapy Enhancement, J Nutr, 2020
