L-Kynurenine visualization in human intestinal immune cells by IF L-Kynurenine visualization in human intestinal immune cells by IF Immunohistochemical analysis reveals accumulation of L-Kynurenine within tumor cells of human colorectal cancer (CRC) tissue. L-Kynurenine detection in human colon cancer tissue by IHC L-Kynurenine detection in human brain tissue by IHC Affinity & Specificity of anti- L-Kynurenine mAb 3D4-F2

L-Kynurenine visualization in human intestinal immune cells by IF

Immunofluorescence staining reveals L-Kynurenine accumulation in specific immune cells in human colon tissue. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/250). After incubation with Alexa-488 conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize IF staining.

L-Kynurenine antibody

Ref: IS003

 

Monoclonal anti-L-Kynurenine antibody 3D4-F2 enabled to illustrate, for the first time, the presence of L-kynurenine in human brain and tumor samples by IHC and IF. Recently, our anti-kynurenine antibody was used to stratify cancer patients for kynurenine pathway activity (open-access PLOS ONE article).

 

Clonality Monoclonal antibody (clone 3D4-F2)
Host Mouse
Validated applications IHC / IF
Specie reactivity Reacts with all species
References 5 citations
Format 50 µL
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Product information Protocols References Technical support Reviews

Product overview

 

Product name L-Kynurenine antibody
Synonyms

(S)-Kynurenine antibody

L-2-Amino-4-(2-aminophenyl)-4-oxobutanoic acid antibody

Kynurenin antibody

3-Anthraniloyl-L-alanine antibody,

Immunogen Conjugated L-Kynurenine
Isotype IgG1 k chain
Clone clone 3D4-F2
Specificity When tested in competitive ELISA, the anti-L-Kynurenine antibody did not show any significant cross reactivity with L-Tryptophan, 3-hydroxy-DL-Kynurenine, Kynurenic acid, Anthranilic acid or 3-hydroxyAnthranilic acid conjugates
Lot number

140201

 

 

Storage

 

Form

Liquid

Purity Purified IgG
Concentration

0,5 mg/ml

Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

Immunohistochemistry (IHC)

Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunofluorescence (IF)

Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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