Cross-validation of L-Lysine ELISA and LC/MS data in human plasma samples
Lys was quantified in human plasma samples from 38 healthy subjects using IS-I-1400 ELISA kit or by LC/MS. Correlation study showed R2=0.986, thereby confirming the accuracy of the immunoassay.
L-Lysine ELISA kitRef: IS-I-1400
L-Lysine (Lys) is an essential amino acid since it's not produced in the body and must be supplied through the diet. It's one of the building blocks for protein, peptide and non-peptide synthesis, and is thus found involved in several physiological and biochemical processes. As a result, Lys deficiency was shown to affect cell cycle, apoptosis, amino acid metabolism, and to even impair antibody responses as well as cell-mediated immune cell functions. In addition, and like other amino acids, Lys contributes to epigenetic and protein regulations and immunosuppression ; its catabolism activation generates acetyl-CoA which, for instance, promotes Wnt signaling and can support tumor growth.
Furthermore, imbalance in circulating Lys levels can be observed in many conditions such as i) inherited diseases on enzymes involved in Lys catabolism (e.g. glutaric aciduria type I or antiquitin deficiency), ii) diabetes-dependent cardiovascular disease, as well as iii) human immunodeficiency viruses-1 infection.
|Range||16 - 650µM (3 - 118µg/mL)
|Assay time||Sample preparation 3h, ELISA overnight|
|Species reactivity||Reacts with all species|
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|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
|Product name||L-Lysine ELISA kit|
|Description||Competitive ELISA kit for the quantitative measurement of L-Lysine (Lys) in plasma samples. For research use only.
|Minimal sample volume||20 µL
|Species reactivity||React with all species|
|Standard range||16 - 650 µM|
No significant cross-reactivity was observed with L-Lysine, Cadaverine dihydrochloride, L-HomoArginine hydrochloride, N-methyl-L-Lysine hydrichloride and N-acetyk-L-Lysine.
|Assay time||Sample preparation 3h and ELISA overnight|
|Storage||Store at 2-8°C for up to 6 months.|
|Datasheets||Instructions for use, Material Safety Datasheet|
For research use only - Do not use for diagnosticTop
|Sample collection & storage||
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation (3 hours)
|ELISA||L-Lysine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
Selected articles on Lysine:
- Yin et al., Effects of Lysine deficiency and Lys-Lys dipeptide on cellular apoptosis and amino acids metabolism, Mol Nutr Food Res, 2017
- Guoyao Wu, Functional amino acids in nutrition and health, Amino Acids, 2013
- Lieu et al., Amino acids in cancer, Exp Mol Med, 2020
- Razquin et al., Lysine pathway metabolites and the risk of type 2 diabetes and cardiovascular disease in the PREDIMED study: results from two case-cohort studies, Cardiovasc Diabetol., 2019
- E. Butorov, Relationship between plasma l-lysine concentrations and levels of HIV-1 RNA, Virulence, 2013