Cross-validation of L-Lysine ELISA and LC/MS data in human plasma samples Cross-validation of L-Lysine ELISA and LC/MS data in human plasma samples Typical standard curve of Lysine ELISA # IS-I-1400 : L-Lysine (Lys) ELISA kit

Cross-validation of L-Lysine ELISA and LC/MS data in human plasma samples

Lys was quantified in human plasma samples from 38 healthy subjects using IS-I-1400 ELISA kit or by LC/MS. Correlation study showed R2=0.986, thereby confirming the accuracy of the immunoassay.

L-Lysine ELISA kit

Ref: IS-I-1400

L-Lysine (Lys) is an essential amino acid since it's not produced in the body and must be supplied through the diet. It's one of the building blocks for protein, peptide and non-peptide synthesis, and is thus found involved in several physiological and biochemical processes. As a result, Lys deficiency was shown to affect cell cycle, apoptosis, amino acid metabolism, and to even impair antibody responses as well as cell-mediated immune cell functions. In addition, and like other amino acids, Lys contributes to epigenetic and protein regulations and immunosuppression ; its catabolism activation generates acetyl-CoA which, for instance, promotes Wnt signaling and can support tumor growth.

Furthermore, imbalance in circulating Lys levels can be observed in many conditions such as i) inherited diseases on enzymes involved in Lys catabolism (e.g. glutaric aciduria type I or antiquitin deficiency), ii) diabetes-dependent cardiovascular disease, as well as iii) human immunodeficiency viruses-1 infection.

 

Sample type
Plasma
Capacity

96 tests

Sensitivity 13µM  (2.4µg/mL)
Range 16 - 650µM  (3 - 118µg/mL)
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species

 

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Product overview

Product name L-Lysine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Lysine (Lys) in plasma samples. For research use only.
Format 96-well plate
Samples Plasma
Minimal sample volume 20 µL
Species reactivity React with all species
Standard range 16 - 650 µM
Sensitivity

13µM

Specificity

No significant cross-reactivity was observed with L-Lysine, Cadaverine dihydrochloride, L-HomoArginine hydrochloride, N-methyl-L-Lysine hydrichloride and N-acetyk-L-Lysine.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  - Do not use for diagnostic

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Key steps

 

Sample collection & storage

EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Lysine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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