Cross-validation of L-Phenylalanine ELISA andand LC/MS data in human plasma samples Cross-validation of L-Phenylalanine ELISA andand LC/MS data in human plasma samples Typical standard curve # IS-I-1700 : L-Phenylalanine (Phe) ELISA kit

Cross-validation of L-Phenylalanine ELISA andand LC/MS data in human plasma samples

L-Phe was quantified in human plasma samples from 40 healthy subjects using IS-I-1700 ELISA kit or by LC/MS. Correlation study showed R2=0.99, thereby confirming the accuracy of the immunoassay.

L-Phenylalanine ELISA kit

Ref: IS-I-1700

L-Phenylalanine (Phe) is an essential amino acid that cannot be synthetized by humans or other vertebrates and must be supplied from an exogenous diet. Phe is transformed to L-Tyrosine (Tyr) through the Phenylalanine hydroxylase (PAH) enzyme and thus serves as a precursor of key catecholamines including Dopamine, Noradrenaline and Adrenaline. Mutations in the gene encoding for PAH have been linked to Phenyloketonuria (PKU), an aminoacidopathies - associated with fair skin, eczema, seizures, tremors, and hyperactivity - characterized by an increased blood/brain level of Phe and its derivatives, and a concomitant decrease in Tyr. Besides its known role in PKU, Phe has been recently linked to severity of COVID-19 where higher level of Phe has been detected for patients with moderate/severe disease in comparison to patients with asymptomatomatic/midly SARS-COV-2 infection (Atila et al, Amino Acids, 2021) thus shedding light on role of Phe on the control of immune response.

 

Sample type
Plasma, Serum
Capacity

96 tests

Sensitivity 1.7µM
Range 3.4 - 390µM
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species
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Product information Key steps References Technical support Reviews

Product overview

Product name L-Phenylalanine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Phenylalanine (Phe) in plasma and serum samples. For research use only.
Format 96-well plate
Samples Plasma, Serum
Minimal sample volume 20 µL
Species reactivity React with all species
Standard range 3.4 - 390 µM
Sensitivity

1.7µM

Specificity

No significant cross-reactivity was observed with L-Tyrosine, L-DOPA, Dopamine hydrochloride or Noradrenaline.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  - Do not use for diagnostic

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Key steps

 

Sample collection & storage

EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Phenylalanine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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References

Selected articles on Phenylalanine:

 

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