Cross-validation of L-Phenylalanine ELISA andand LC/MS data in human plasma samples Cross-validation of L-Phenylalanine ELISA andand LC/MS data in human plasma samples Typical standard curve # IS-I-1700 : L-Phenylalanine (Phe) ELISA kit

Cross-validation of L-Phenylalanine ELISA andand LC/MS data in human plasma samples

L-Phe was quantified in human plasma samples from 40 healthy subjects using IS-I-1700 ELISA kit or by LC/MS. Correlation study showed R2=0.99, thereby confirming the accuracy of the immunoassay.

L-Phenylalanine ELISA kit

Ref: IS-I-1700

L-Phenylalanine (Phe) is an essential amino acid that cannot be synthetized by humans or other vertebrates and must be supplied from an exogenous diet. Phe is transformed to L-Tyrosine (Tyr) through the Phenylalanine hydroxylase (PAH) enzyme and thus serves as a precursor of key catecholamines including Dopamine, Noradrenaline and Adrenaline. Mutations in the gene encoding for PAH have been linked to Phenyloketonuria (PKU), an aminoacidopathies - associated with fair skin, eczema, seizures, tremors, and hyperactivity - characterized by an increased blood/brain level of Phe and its derivatives, and a concomitant decrease in Tyr. Besides its known role in PKU, Phe has been recently linked to severity of COVID-19 where higher level of Phe has been detected for patients with moderate/severe disease in comparison to patients with asymptomatomatic/midly SARS-COV-2 infection (Atila et al, Amino Acids, 2021) thus shedding light on role of Phe on the control of immune response.


Sample type
Plasma, Serum

96 tests

Sensitivity 1.7µM
Range 3.4 - 390µM
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species
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Product overview

Product name L-Phenylalanine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Phenylalanine (Phe) in plasma and serum samples. For research use only.
Format 96-well plate
Samples Plasma, Serum
Minimal sample volume 20 µL
Species reactivity React with all species
Standard range 3.4 - 390 µM



No significant cross-reactivity was observed with L-Tyrosine, L-DOPA, Dopamine hydrochloride or Noradrenaline.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet


For research use only  - Do not use for diagnostic


Key steps


Sample collection & storage

EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Phenylalanine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use


Selected articles on Phenylalanine:



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