Serotonin ELISA kit
Ultra-Sensitive – Any sample

Ref: BA-E-5900R
NeuromediatorsTryptophan metabolismResearch ELISA kits (RUO)ELISA

This Serotonin (5-HT) ELISA kit enables the ultra-sensitive measurement of 5-hydroxytryptamine in any biological sample. Intended for research use only, the assay features a lower limit of detection at 5pg/ml and a minimal sample volume as low as 1µl.

Sample type 1 – 100µl – any sample
Samples/kit 40 samples in duplicate
Sensitivity 5pg/ml
Range 0.015 – 2.5ng/ml
Assay time 30 min sample preparation, ELISA overnight
Reactivity Reacts with all species
Citations Cited in over 20 papers

1 x 96 wells


32 in stock



Worldwide shipping

Select delivery location

Product overview

Product name Serotonin ELISA kit – Research
Description Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Serotonin in biological samples. Research use only
Format 96-well plate
Samples 1 – 100µl – any biological sample
Reactivity Reacts with all species
Standard range 0 / 0.015 – 2.5ng/ml


Specificity No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan
Assay time 30 min + overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial safety datasheet

Sample preparation Sample acylation (30min)
ELISA Serotonin antiserum incubation (overnight), revelation and read steps (1h)
Detailed protocol Download instructions for use

Product citation

  • Sugarcane (Saccharum officinarum L.) Top Extract Ameliorates Cognitive Decline in Senescence Model SAMP8 Mice: Modulation of Neural Development and Energy Metabolism
    Check article
    Authors : Iwata et al., Frontiers in Cell and Developmental Biology
  • Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress
    Check article
    Authors : Letenneur et al., Nutrients
  • Unlocking the Secrets: Exploring the Biochemical Correlates of Suicidal Thoughts and Behaviors in Adults with Autism Spectrum Conditions
    Check the article
    Authors : Cremone et al., Biomedicines
  • Transcriptomics and biochemical evidence of trigonelline ameliorating learning and memory decline in the senescence-accelerated mouse prone 8 (SAMP8) model by suppressing proinflammatory cytokines and elevating neurotransmitter release
    Check article
    Authors : Aktar et al., GeroScience
  • Paraburkholderia sabiae administration alters zebrafish anxiety-like behavior via gut microbial taurine metabolism
    Check the article
    Authors : Ichikawa et al., Frontiers in Microbiology
  • Serum serotonin levels are elevated in patients with increased risk of rheumatoid arthritis
    Check the article
    Authors : Wirestam et al., Frontiers in Medicine
  • Microalgae extract induces antidepressant-like activity via neuroinflammation regulation and enhances the neurotransmitter system
    Check the article
    Authors : Sasaki et al., Food & Chemical Toxicology
  • Histamine via histamine H1 receptor enhances the muscarinicreceptor-induced calcium response to acetylcholine in anenterochromaffin cell model
    Check the article
    Authors : Pfanzagl et al., Clinical & Experimental Pharmacology & Physiology
  • The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)
    Check the article
    Authors : Francikowski et al., Scientific reports
  • Antidepressant- and anxiolytic-like activities of Rosmarinus officinalis extract in rodent models: Involvement of oxytocinergic system
    Check the article
    Authors : Sasaki et al., Biomedicine & Pharmacotherapy
  • Neurobehavioral effects of acute low-dose whole-body irradiation
    Check the article
    Authors : Bekal et al., Journal of radiation research
  • Luteolin Modulates Neural Stem Cells Fate Determination: In vitro Study on Human Neural Stem Cells, and in vivo Study on LPS-Induced Depression Mice Model
    Check the article
    Authors : Achour et al., Frontiers in cell & developmental biology
  • Improvement of loperamide-induced slow transit constipation by Bifidobacterium bifidum G9-1 is mediated by the correction of butyrate production and neurotransmitter profile due to improvement in dysbiosis
    Check the article
    Authors : Makizaki et al., PloS one
  • Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression
    Check the article
    Authors : Sabti et al., International Journal of Molecular Sciences
  • Serotonin signaling regulates insulin-like peptides for growth, reproduction, and metabolism in the disease vector Aedes aegypti
    Check the article
    Authors : Ling et al., Proceedings of the National Academy of Sciences of the USA
  • Ketamine treatment involves medial prefrontal cortex serotonin to induce a rapid antidepressant-like activity in BALB/cJ mice
    Check the article
    Authors : Pham et al., Neuropharmacology
  • The presence of serotonin in cigarette smoke – a possible mechanistic link to 5-HT-induced airway inflammation
    Check the article
    Authors : Lau et al., Free radical research
  • Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo
    Check the article
    Authors : Kaushal et al., European Neuropsychopharmacology
  • The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease
    Check the article
    Authors : Dammen et al., PloS one
  • Contribution of systemic factors in the pathophysiology of repeated blast-induced neurotrauma
    Check the article
    Authors : Valiyaveettil et al., Neuroscience letters
  • Exploring the role of 5-HT1A receptors in the regulation of prepulse inhibition in mice: implications for cross-species comparisons
    Check the article
    Authors : Van den Buuse et al., ACS chemical neuroscience
  • Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies
    Check the article
    Authors : Rokstad et al., Cancer science
  • The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion
    Check the article
    Authors : Chin et al., American journal of physiology, gastrointestinal & liver physiology

You may also like…

Tryptophan ELISA kit high sensitivity

L-Tryptophan ELISA kit
Any sample I High Sensitivity


Use In Vitro Diagnostics / Research
Samples ≥ 20µL Biological fluids
Key Feature High Sensitivity

Serotonin Antibody – Rabbit Polyclonal


Used for IF in mouse primary neurons and brain tissues, as well as crayfish brain tissues

From: 440.00

Dopamine ELISA kit – Ultra-Sensitive
Any sample


Use Research only
Samples Any type, ≥ 1µl
Key Feature High Sensitivity


5-Hydroxyindoleacetic acid (5-HIAA) ELISA kit
Fast – Urine samples


Use In Vitro Diagnostics, Research
Samples Urine ≥50µl
Feature Fast (3h)

Serotonin ELISA kit
I Fast Assay I Serum and Urine


Use In Vitro Diagnostics, Research
Samples Urine, plasma ≥25µl
Feature Fast – 75 min