Antibodies & immunoassays for small molecules Serotonin ELISA kit
Any sample
Ref: BA-E-5900R
NeuromediatorsTryptophan metabolismResearch ELISA kits (RUO)ELISA
+Serotonin ELISA kit
DatasheetMSDSProtocol
This Serotonin (5-HT) ELISA kit enables the ultra-sensitive measurement of 5-hydroxytryptamine in any biological sample. Intended for research use only, the assay features a lower limit of detection at 5pg/ml and a minimal sample volume as low as 1µl.
| Sample type | 1 – 100µl – any sample |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 5pg/ml |
| Range | 0.015 – 2.5ng/ml |
| Assay time | 30 min sample preparation, ELISA overnight |
| Reactivity | Reacts with all species |
| Citations | Over 20 |
Product overview
| Product name | Serotonin ELISA kit – Research |
| Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Serotonin in biological samples. Research use only |
| Format | 96-well plate |
| Samples | 1 – 100µl – any biological sample |
| Reactivity | Reacts with all species |
| Standard range | 0 / 0.015 – 2.5ng/ml |
| Sensitivity |
5pg/ml |
| Specificity | No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan |
| Assay time | 30 min + overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| Sample preparation | Sample acylation (30min) |
| ELISA | Serotonin antiserum incubation (overnight), revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Microalgae extract induces antidepressant-like activity via neuroinflammation regulation and enhances the neurotransmitter system
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Authors : Sasaki et al., Food & Chemical Toxicology
2022-11 - Histamine via histamine H1 receptor enhances the muscarinicreceptor-induced calcium response to acetylcholine in anenterochromaffin cell model
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Authors : Pfanzagl et al., Clinical & Experimental Pharmacology & Physiology
2022-06 - The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)
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Authors : Francikowski et al., Scientific reports
2021-10 - Antidepressant- and anxiolytic-like activities of Rosmarinus officinalis extract in rodent models: Involvement of oxytocinergic system
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Authors : Sasaki et al., Biomedicine & Pharmacotherapy
2021-10 - Neurobehavioral effects of acute low-dose whole-body irradiation
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Authors : Bekal et al., Journal of radiation research
2021-09 - Luteolin Modulates Neural Stem Cells Fate Determination: In vitro Study on Human Neural Stem Cells, and in vivo Study on LPS-Induced Depression Mice Model
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Authors : Achour et al., Frontiers in cell & developmental biology
2021-08 - Improvement of loperamide-induced slow transit constipation by Bifidobacterium bifidum G9-1 is mediated by the correction of butyrate production and neurotransmitter profile due to improvement in dysbiosis
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Authors : Makizaki et al., PloS one
2021-03 - Elucidation of the Molecular Mechanism Underlying Lippia citriodora(Lim.)-Induced Relaxation and Anti-Depression
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Authors : Sabti et al., International Journal of Molecular Sciences
2019-07 - Serotonin signaling regulates insulin-like peptides for growth, reproduction, and metabolism in the disease vector Aedes aegypti
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Authors : Ling et al., Proceedings of the National Academy of Sciences of the USA
2018-10 - Ketamine treatment involves medial prefrontal cortex serotonin to induce a rapid antidepressant-like activity in BALB/cJ mice
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Authors : Pham et al., Neuropharmacology
2016-05 - The presence of serotonin in cigarette smoke – a possible mechanistic link to 5-HT-induced airway inflammation
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Authors : Lau et al., Free radical research
2016-03 - Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo
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Authors : Kaushal et al., European Neuropsychopharmacology
2013-08 - The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease
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Authors : Dammen et al., PloS one
2013-04 - Contribution of systemic factors in the pathophysiology of repeated blast-induced neurotrauma
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Authors : Valiyaveettil et al., Neuroscience letters
2013-01 - Exploring the role of 5-HT1A receptors in the regulation of prepulse inhibition in mice: implications for cross-species comparisons
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Authors : Van den Buuse et al., ACS chemical neuroscience
2012-12 - Microencapsulation of small intestinal neuroendocrine neoplasm cells for tumor model studies
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Authors : Rokstad et al., Cancer science
2012-04 - The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion
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Authors : Chin et al., American journal of physiology, gastrointestinal & liver physiology
2011-10
