IHC validation of anti-3-Hydroxy-Anthranilic acid antibody in human colon tumor IHC validation of anti-3-Hydroxy-Anthranilic acid antibody in human colon tumor IHC validation of 3-HydroxyAnthranilic acid antibody in human brain tissue Affinity & specificity of the 3-HydroxyAnthranilic acid antibody 3-Hydroxy-Anthranilic acid

IHC validation of anti-3-Hydroxy-Anthranilic acid antibody in human colon tumor

IHC staining shows accumulation of 3-hydroxy-anthranilic acid in human colon cancer cells, as well as in cells from the tumoral microenvironment. Human paraffin-embedded colon tumor tissue was subjected to pH=6 antigen retrieval, and overnight incubation with primary 3-Hydroxy-Anthranilic acid antibody (1/1000 dilution). After polymer-conjugated secondary antibody exposure, staining was observed through DAB coloration.

3-HydroxyAnthranilic acid antibody

Ref: IS001

 

The first and only anti-3-HydroxyAnthranilic acid antibody available for research use. The 5B2-G2 primary mouse monoclonal antibody was validated for IHC both in human tumor and brain tissues. When tested by competitive ELISA, the antibody demonstrated strong affinity and high specificity.

 

Clonality Monoclonal antibody (clone 5B2-G2)
Host Mouse
Valided applications IHC
Specie reactivity Reacts with all species
References Not yet cited to our knowledge
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Product information Protocols References Technical support Reviews

Product overview

 

Product name 3-Hydroxyanthranilic acid antibody
Synonyms

Anti-3-Hydroxy-Anthranilic acid antibody

2-Amino-3-hydroxybenzoic acid antibody

3-OH-Anthranilic acid antibody

3-hydroxanthranilate antibody

3-OHAA antibody

Immunogen Conjugated 3-Hydroxyanthranilic acid
Isotype IgG1 k chain
Clone Clone 5B2-G2
Specificity When tested in competitive ELISA, the anti- 3-HydroxyAnthranilic acid antibody did not show any significant cross reactivity with Anthranilic acid or Cinnabarinic acid conjugates
Lot number

140201

Expiration date April 2022

 

 

Storage

Form

Liquid

Purity Purified IgG
Concentration

0,5 mg/ml

Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

Immunohistochemistry (IHC)

Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunofluorescence (IF)

Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent dyes conjugated secondary antibody

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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References

Antibody not yet cited. Submit an article and get a 10% discount.

 

Selected publications on 3-HydroxyAnthranilic acid:

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