IHC validation of anti-3-Hydroxy-Anthranilic acid antibody in human colon tumor
IHC staining shows accumulation of 3-hydroxy-anthranilic acid in human colon cancer cells, as well as in cells from the tumoral microenvironment. Human paraffin-embedded colon tumor tissue was subjected to pH=6 antigen retrieval, and overnight incubation with primary 3-Hydroxy-Anthranilic acid antibody (1/1000 dilution). After polymer-conjugated secondary antibody exposure, staining was observed through DAB coloration.
3-HydroxyAnthranilic acid antibodyRef: IS001
The first and only anti-3-HydroxyAnthranilic acid antibody available for research use. The 5B2-G2 primary mouse monoclonal antibody was validated for IHC both in human tumor and brain tissues. When tested by competitive ELISA, the antibody demonstrated strong affinity and high specificity.
|Clonality||Monoclonal antibody (clone 5B2-G2)|
|Specie reactivity||Reacts with all species|
|References||Not yet cited to our knowledge
Submit content and get a 10% discount!
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
|Product name||3-Hydroxyanthranilic acid antibody|
Anti-3-Hydroxy-Anthranilic acid antibody
2-Amino-3-hydroxybenzoic acid antibody
3-OH-Anthranilic acid antibody
|Immunogen||Conjugated 3-Hydroxyanthranilic acid
|Isotype||IgG1 k chain|
|Specificity||When tested in competitive ELISA, the anti- 3-HydroxyAnthranilic acid antibody did not show any significant cross reactivity with Anthranilic acid or Cinnabarinic acid conjugates
|Expiration date||April 2022|
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent dyes conjugated secondary antibody
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only|
Antibody not yet cited. Submit an article and get a 10% discount.
Selected publications on 3-HydroxyAnthranilic acid:
- Lee WS, Lee SM, Kim MK, Park SG, Choi IW, Choi I, Joo YD, Park SJ, Kang SW, Seo SK. Int Immunopharmacol. The tryptophan metabolite 3-hydroxyanthranilic acid suppresses T cell responses by inhibiting dendritic cell activation. 2013 Nov;17(3):721-6. doi: 10.1016/j.intimp.2013.08.018. Epub 2013 Sep 9.
- Adams S, Braidy N, Bessede A, Brew BJ, Grant R, Teo C, Guillemin GJ. The kynurenine pathway in brain tumor pathogenesis. Cancer Res. 2012 Nov 15;72(22):5649-57. doi: 10.1158/0008-5472.CAN-12-0549. Epub 2012 Nov 9.
- Krause D, Suh HS, Tarassishin L, Cui QL, Durafourt BA, Choi N, Bauman A, Cosenza-Nashat M, Antel JP, Zhao ML, Lee SC. The tryptophan metabolite 3-hydroxyanthranilic acid plays anti-inflammatory and neuroprotective roles during inflammation: role of hemeoxygenase-1. Am J Pathol. 2011 Sep;179(3):1360-72. doi: 10.1016/j.ajpath.2011.05.048.
- Yan Y, Zhang GX, Gran B, Fallarino F, Yu S, Li H, Cullimore ML, Rostami A, Xu H. IDO upregulates regulatory T cells via tryptophan catabolite and suppresses encephalitogenic T cell responses in experimental autoimmune encephalomyelitis. J Immunol. 2010 Nov 15;185(10):5953-61. doi: 10.4049/jimmunol.1001628. Epub 2010 Oct 13.
- Hayashi T, Mo JH, Gong X, Rossetto C, Jang A, Beck L, Elliott GI, Kufareva I, Abagyan R, Broide DH, Lee J, Raz E. 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18619-24. Epub 2007 Nov 14.