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Arginine ELISA kit – High Sensitivity – Plasma, Serum, Supernatant
Ref: IS-I-0400RArginine metabolismAmino acidsResearch ELISA kits (RUO)ELISA
+L-Arginine ELISA kit
DatasheetProtocolMSDS
Arginine (Arg) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.
The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.
| Sample type | Serum, Plasma, Cell culture |
| Capacity | 96 tests |
| Sensitivity | 2.1µM |
| Range | 13 – 500µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
| References | Cited in 2 papers |
Product overview
| Product name | L-Arginine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only |
| Format | 96-well plate |
| Samples | Serum, Plasma, Cell culture |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 5.12 – 200µM |
| Sensitivity | 2.1µM |
| Specificity | No significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA |
| Assay time | Sample preparation 3h and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Arginine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Lactobacillus plantarum ameliorates NASH-related inflammation by upregulating L-arginine production
Check the article
Authors : Kim et al., Experimental & Molecular Medicine
2023-11 - Circulating L-Arginine predicts the survival of cancer patients treated with immune checkpoint inhibitors
Check the article
Authors : Peyraud et al., Annals of oncology
2022-07
