Anthranilic acid detection by IHC in human brain Anthranilic acid detection by IHC in human brain Affinity & specificity of anti-Anthranilic acid antibody Anthranilic acid

Anthranilic acid detection by IHC in human brain

Immunohistochemical analysis of human caudate-putamen highlights Anthranilic acid accumulation in the cytoplasm of glial cells. Paraffin-embedded brain tissue was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/50). After incubation with polymer conjugated secondary Ab, DAB was used to reveal the staining.

Anthranilic acid antibody

Ref: IS013

 

The monoclonal anti- Anthranilic acid antibody 10G1-F1 was validated for immunohistochemistry (IHC) in human brain tissues. Highly specific, as demonstrated by competitive ELISA, the antibody consistently delivered clean and reproducible stainings.

 

Clonality Monoclonal antibody (clone 10G1-F1)
Host Mouse
Valided applications IHC
Specie reactivity Reacts with all species
References Not yet cited to our knowledge
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Product information Protocols References Technical support Reviews

Product overview

 

Product name Anthranilic acid antibody
Synonyms

2-AA antibody

Anti- vitamin L1 antibody

Anti- Anthranilate antibody

Anthranilate antibody

2-Aminobenzoic acidantibody

Immunogen Conjugated Anthranilic acid
Clone 10G1-F1
Specificity When tested in competitive ELISA, the anti-anthranilic antibody did not show any significant cross reactivity with 3-hydroxyAnthranilic acid
Lot number

140201

 

Storage

 

Form

Liquid

Purity Purified IgG
Concentration

0,5 mg/ml

Storage Store at 4°C
Storage buffer

Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles

Material safety datasheet Download MSDS
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Protocols

Immunohistochemistry (IHC)

Dilute at 1:50-1:500. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunofluorescence (IF)

Dilute at 1:50-1:500 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with incubate with fluorescent secondary antibody conjugate

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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