ELISA Arginine quantification in mouse 4T1 tumor and non-tumor microdialysates
Mice were implanted with 4T1 breast tumor cells and then exposed to either PD1 or CTLA4 blockade treatment each alone or in combination. They were then processed for tumoral vs. non- tumoral microdialysis sampling for the determination of L-Arginine levels, among other metabolites. ELISA-based quantification of L- Arginine (using IS-I-0400 kit) highlights the lower arginine level within the tumor microenvironment with respect to non-tumor compartment – underlining tumor arginine consumption. Also, this level seems not to be modulated upon single anti-PD1 or anti- CTLA4 agents – the 4T1 model being known as non-responsive to these monotherapies, while PD1/CTLA4 blockade combination is shown to bring this level back to that in the non-tumor compartment.
Arginine ELISA kitRef: IS-I-0400
Arginine (ARG) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.
The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.
||Serum, Plasma, Cell culture
|Range||5.12 - 200µM|
|Assay time||Sample preparation 3h, ELISA overnight|
|Species reactivity||Reacts with all species|
Shipping info :
(for 1 kit)
|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
|Product name||L-Arginine ELISA kit|
|Description||Competitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only.
|Samples||Serum, Plasma, Cell culture|
|Minimal sample volume||20 µL
|Species reactivity||React with all species|
|Standard range||5.12 - 200 µM|
No significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA.
|Assay time||Sample preparation 3h and ELISA overnight|
|Storage||Store at 2-8°C for up to 6 months.|
|Datasheets||Instructions for use, Material Safety Datasheet|
For research use only - Do not use for diagnosticTop
|Sample collection & storage||
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation (3 hours)
|ELISA||L-Arginine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
Selected articles on L-Arginine:
- Satohet al., Supplementation of L-arginine boosts the therapeutic efficacy of anticancer chemoimmunotherapy, Cancer Sci. 2020 May 19
- Grosse et al., Arginine Derivatives in Cerebrovascular Diseases: Mechanisms and Clinical Implications, Int J Mol Sci. 2020 Mar 5
- Heffernan et al., L-Arginine as a Nutritional Prophylaxis Against Vascular Endothelial Dysfunction With Aging, J Cardiovasc Pharmacol Ther. 2010 Mar.
- Boget et al., L-Arginine Improves Vascular Function by Overcoming Deleterious Effects of ADMA, a Novel Cardiovascular Risk Factor, Altern. Med Rev, 2005 Mar.