PI3K signaling pathway inhibition limits Arginase 1 activity of M2 polarized macrophages PI3K signaling pathway inhibition limits Arginase 1 activity of M2 polarized macrophages Arginine quantification in human plasma samples Arginine quantification in Tumor/Non Tumor microdialysate samples Cross-validation of Arginine ELISA and LC/MS data in human plasma samples Typical standard curve of Arginine ELISA

PI3K signaling pathway inhibition limits Arginase 1 activity of M2 polarized macrophages

During their polarization, M2 macrophages were treated with increasing concentrations of LY294002, a reference PI3K inhibitor. ELISA-based quantification of L-Arginine increase in the supernatants (using IS-I-0400 kit) highlighted a dose-dependent inhibition of Arginine catabolism.

Arginine ELISA kit

Ref: IS-I-0400

Arginine (ARG) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.

The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.

 

Sample type
Serum, Plasma, Cell culture
Capacity

96 tests

Sensitivity 2.1µM
Range 5.12 - 200µM
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species

 

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Product overview

Product name L-Arginine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only.
Format 96-well plate
Samples Serum, Plasma, Cell culture
Minimal sample volume 20 µL
Species reactivity React with all species
Standard range 5.12 - 200 µM
Sensitivity

2.1µM

Specificity

No significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets  Instructions for useMaterial Safety Datasheet

 

For research use only  - Do not use for diagnostic

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Key steps

Sample collection & storage

Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Arginine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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