PI3K signaling pathway inhibition limits Arginase 1 activity of M2 polarized macrophages
During their polarization, M2 macrophages were treated with increasing concentrations of LY294002, a reference PI3K inhibitor. ELISA-based quantification of L-Arginine increase in the supernatants (using IS-I-0400 kit) highlighted a dose-dependent inhibition of Arginine catabolism.
Arginine ELISA kitRef: IS-I-0400
Arginine (ARG) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.
The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.
||Serum, Plasma, Cell culture
|Range||5.12 - 200µM|
|Assay time||Sample preparation 3h, ELISA overnight|
|Species reactivity||Reacts with all species|
Shipping info :
(for 1 kit)
|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
|Product name||L-Arginine ELISA kit|
|Description||Competitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only.
|Samples||Serum, Plasma, Cell culture|
|Minimal sample volume||20 µL
|Species reactivity||React with all species|
|Standard range||5.12 - 200 µM|
No significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA.
|Assay time||Sample preparation 3h and ELISA overnight|
|Storage||Store at 2-8°C for up to 6 months.|
|Datasheets||Instructions for use, Material Safety Datasheet|
For research use only - Do not use for diagnosticTop
|Sample collection & storage||
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation (3 hours)
|ELISA||L-Arginine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
Selected articles on L-Arginine:
- Satohet al., Supplementation of L-arginine boosts the therapeutic efficacy of anticancer chemoimmunotherapy, Cancer Sci. 2020 May 19
- Grosse et al., Arginine Derivatives in Cerebrovascular Diseases: Mechanisms and Clinical Implications, Int J Mol Sci. 2020 Mar 5
- Heffernan et al., L-Arginine as a Nutritional Prophylaxis Against Vascular Endothelial Dysfunction With Aging, J Cardiovasc Pharmacol Ther. 2010 Mar.
- Boget et al., L-Arginine Improves Vascular Function by Overcoming Deleterious Effects of ADMA, a Novel Cardiovascular Risk Factor, Altern. Med Rev, 2005 Mar.