ELISA Arginine quantification in mouse 4T1 tumor and non-tumor microdialysates ELISA Arginine quantification in mouse 4T1 tumor and non-tumor microdialysates PI3K signaling pathway inhibition limits Arginase 1 activity of M2 polarized macrophages Arginine quantification in human plasma samples Cross-validation of Arginine ELISA and LC/MS data in human plasma samples Typical standard curve of Arginine ELISA

ELISA Arginine quantification in mouse 4T1 tumor and non-tumor microdialysates

Mice were implanted with 4T1 breast tumor cells and then exposed to either PD1 or CTLA4 blockade treatment each alone or in combination. They were then processed for tumoral vs. non- tumoral microdialysis sampling for the determination of L-Arginine levels, among other metabolites. ELISA-based quantification of L- Arginine (using IS-I-0400 kit) highlights the lower arginine level within the tumor microenvironment with respect to non-tumor compartment – underlining tumor arginine consumption. Also, this level seems not to be modulated upon single anti-PD1 or anti- CTLA4 agents – the 4T1 model being known as non-responsive to these monotherapies, while PD1/CTLA4 blockade combination is shown to bring this level back to that in the non-tumor compartment.

Arginine ELISA kit

Ref: IS-I-0400

Arginine (ARG) is a basic non-essential amino acid well known to participate in physiological functions such as i) vascular tone homeostasis as contributing to the production of nitric oxide (NO) and ii) immune response control, through NO synthase (NOS) and Arginase enzymes, respectively.

The Arginine (Arg) ELISA kit allows for the determination of Arg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.

 

Sample type
Serum, Plasma, Cell culture
Capacity

96 tests

Sensitivity 2.1µM
Range 5.12 - 200µM
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species


615
Indicative exchange rate
Contact sales
cb
cb
Shipping info :
(for 1 kit)
54.00€ , 5 days
Product information Key steps References Technical support Reviews

Product overview

Product name L-Arginine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of Arginine (L-Arg) in serum, plasma and cell culture samples. For research use only.
Format 96-well plate
Samples Serum, Plasma, Cell culture
Minimal sample volume 20 µL
Species reactivity React with all species
Standard range 5.12 - 200 µM
Sensitivity

2.1µM

Specificity

No significant cross-reactivity was observed with HomoArginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets  Instructions for useMaterial Safety Datasheet

 

For research use only  - Do not use for diagnostic

Top

Key steps

Sample collection & storage

Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Arginine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
Top

Technical Support Submit a question

No technical question yet. Be the first to ask! Top

Reviews Submit your review

No reviews yet. Thanks for rating this product. Top
Any question?

Just ask us. We provide technical support within 24h.