Gamma-aminobutyric acid ELISA Gamma-aminobutyric acid ELISA

Gamma-aminobutyric acid ELISA

Directly synthesized from Glutamic acid to limit neuronal excitability, Gamma-aminobutyric acid (GABA) is found at concentrations of 1-10 mM in the mammalian brain. Although GABA is mainly known for its inhibitory function, the molecule can exhibit excitatory functions, as in insects, through the activation of cation currents, and in the mammalian developing brain, where it can excite immature neurons.

GABA ELISA kit - Research

Ref: BA-E-2500

 

This GABA ELISA kit enables the measurement of Gamma-aminobutyric acid in any biological sample. Intended for research use only, this quantitative assay can be performed in cell cultures, tissue homogenates, urine, plasma, serum, etc.

 

Sample type
100 - 300 µl, all biological samples
Samples/kit 40 samples in duplicate
Sensitivity

Depending on sample matrix and volume

Range 0 / 25 - 2 500 ng/ml, 0 / 75 – 7 500 ng/ml
Assay time Extraction & derivatization (2h30), ELISA overnight
Species reactivity Reacts with all species
References Citations in litterature
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Product information Key steps References Technical support Reviews

Product overview

Product name

GABA ELISA kit - Research

Description Enzyme immunoassay (ELISA) for the quantitative determination of γ-Aminobutyric acid in biological samples. Research use only.
Format 96-well plate
Samples Any sample
Sample volume 100 - 300 µl
Cross specie-reactivity React with all species
Standard range serum, plasma 0 / 25 - 2 500 ng/ml
Sensitivity

Depending on sample matrix and volume

Specificity No significant cross-reactivity was observed with GABA analogs such as Beta-Alanine and alpha-aminobutyric acid
Assay time 2h30 sample extraction and derivatization, ELISA overnight
Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample preparation

Sample extraction and derivatization (2h30)

ELISA GABA antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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