Gamma-aminobutyric acid ELISA
Directly synthesized from Glutamic acid to limit neuronal excitability, Gamma-aminobutyric acid (GABA) is found at concentrations of 1-10 mM in the mammalian brain. Although GABA is mainly known for its inhibitory function, the molecule can exhibit excitatory functions, as in insects, through the activation of cation currents, and in the mammalian developing brain, where it can excite immature neurons.
GABA ELISA kit - Research
Ref: BA-E-2500
This GABA ELISA kit enables the measurement of Gamma-aminobutyric acid in any biological sample. Intended for research use only, this quantitative assay can be performed in cell cultures, tissue homogenates, urine, plasma, serum, etc.
| Sample type |
100 - 300 µl, all biological samples |
| Samples/kit | 40 samples in duplicate |
| Sensitivity |
Depending on sample matrix and volume |
| Range | 0 / 25 - 2 500 ng/ml, 0 / 75 – 7 500 ng/ml |
| Assay time | Extraction & derivatization (2h30), ELISA overnight |
| Species reactivity | Reacts with all species |
| References | Citations in litterature |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
GABA ELISA kit - Research |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of γ-Aminobutyric acid in biological samples. Research use only. |
| Format | 96-well plate |
| Samples | Any sample |
| Sample volume | 100 - 300 µl |
| Cross specie-reactivity | React with all species |
| Standard range | serum, plasma 0 / 25 - 2 500 ng/ml |
| Sensitivity |
Depending on sample matrix and volume |
| Specificity | No significant cross-reactivity was observed with GABA analogs such as Beta-Alanine and alpha-aminobutyric acid |
| Assay time | 2h30 sample extraction and derivatization, ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation |
Sample extraction and derivatization (2h30) |
| ELISA | GABA antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Product citation: Proctor et al. Electrophoretic drug delivery for seizure control. Sci Adv. 2018 Aug 29.
- Product citation: Uguzet al. A Microfluidic Ion Pump for In Vivo Drug Delivery. Adv Mater. 2017 Jul;29
- Miraucourt LS et al. GABA expression and regulation by sensory experience in the developing visual system. PLoS One. 2012;7(1):e29086.
- El-Ansary AK, Ben Bacha A, Kotb M. Etiology of autistic features: the persisting neurotoxic effects of propionic acid. J Neuroinflammation. 2012 Apr 24;9:74.
- Al-Wadei HA, Al-Wadei MH, Masi T, Schuller HM. Chronic exposure to estrogen and the tobacco carcinogen NNK cooperatively modulates nicotinic receptors in small airway epithelial cells. Lung Cancer. 2010 Jul;69(1):33-9.
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