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The L-Kynurenine ELISA: A cell-based assay to screen IDO/TDO inhibitors

ImmuSmol’s L-Kynurenine ELISA kit BA-E-2200 can be used to screen IDO/TDO inhibitors in cell lines. Below an example with IDO inhibitor 1-Methyl-L-Tryptophan.

IDO - TDO inhibition cell-based assay screening

Measurement of IDO inhibition by 1-Methyl-L-Tryptophan in cancer cell line. IDO+ CT26 colorectal cancer cell line was exposed to increasing concentrations of 1-Methyl-L-Tryptophan. Kynurenine levels were measured in 20µL cell culture supernatant using ImmuSmol’s ELISA kit BA-E-2200.