Determination of L-Kynurenine levels in cell cultures through microdialysis collection and by ELISA quantification
Murine CT26 colorectal cancer cell line WT or stably transfected with vector containing cDNA encoding for indoleamine 2,3 dioxygenase (IDO1) or Human U-87 MG glioblastoma cell line were cultured for 48 hours. Cell culture was subjected to microdialysis collection (3 fractions collected) and kynurenine was measured using the Kynurenine ELISA kit (BA-E-2200). As expected, kynurenine level was higher in IDO1-proficient CT26 cells than in parental (WT) CT26 cell lines, and also high in U87-MG cells.
L-Kynurenine ELISA kit
Ref: BA-E-2200
The L-Kynurenine ELISA is an easy-to-use assay tailored for the quantification of Kynurenine across large series of samples. Optimized for the analysis of biological fluids (plasma, cell culture supernatant, serum...), the KYN ELISA features a lower limit of detection at 45.7 ng/ml and a minimal sample volume of 20µl, thus allowing longitudinal studies in mice.
| Sample type |
Plasma, serum, cell culture supernatant |
| Capacity | 96 tests |
| Sensitivity |
< 45.7 ng/ml |
| Range | 0/100 - 10000 ng/ml |
| Assay time | Sample acylation 90 min, ELISA overnight |
| Species reactivity | Reacts with all species |
| References | Citations in litterature |
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Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
L-Kynurenine ELISA kit |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant. |
| Format | 96-well plate |
| Samples | Plasma, serum, cell culture supernatant |
| Minimal sample volume | 20 µL |
| Cross specie-reactivity | React with all species |
| Standard range | 0/100 - 10000 ng/ml |
| Sensitivity |
< 47.5 ng/ml |
| Specificity |
No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid. |
| Assay time | Sample acylation 90 min and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
| Sample collection & storage |
Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation |
Sample acylation (90 min) |
| ELISA | L-Kynurenine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
Product citations :
- Klar et al. A highly efficient modality to block the degradation of tryptophan for cancer immunotherapy: locked nucleic acid-modified antisense oligonucleotides to inhibit human indoleamine 2,3-dioxygenase 1/tryptophan 2,3-dioxygenase expression. Cancer Immunol Immunother. 2020 Jan
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