Determination of L-Kynurenine levels in cell cultures through microdialysis collection and by ELISA quantification Determination of L-Kynurenine levels in cell cultures through microdialysis collection and by ELISA quantification Kynurenine ELISA cross-validation by GC/MS in human plasma samples L-Kynurenine quantification by ELISA in 20µl mice plasma samples Determination of L-Kynurenine levels in CT26 cell cultures by ELISA Kynurenine ELISA – typical standard curve

Determination of L-Kynurenine levels in cell cultures through microdialysis collection and by ELISA quantification

Murine CT26 colorectal cancer cell line WT or stably transfected with vector containing cDNA encoding for indoleamine 2,3 dioxygenase (IDO1) or Human U-87 MG glioblastoma cell line were cultured for 48 hours. Cell culture was subjected to microdialysis collection (3 fractions collected) and kynurenine was measured using the Kynurenine ELISA kit (BA-E-2200). As expected, kynurenine level was higher in IDO1-proficient CT26 cells than in parental (WT) CT26 cell lines, and also high in U87-MG cells.

L-Kynurenine ELISA kit

Ref: BA-E-2200
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The L-Kynurenine ELISA is an easy-to-use assay tailored for the quantification of Kynurenine across large series of samples. Optimized for the analysis of biological fluids (plasma, cell culture supernatant, urine...), the KYN ELISA features a lower limit of detection at 47.5 ng/ml and a minimal sample volume of 20µl, thus allowing longitudinal studies in mice.

 

Sample type
Plasma, serum, cell culture supernatant, urine
Capacity 96 tests
Sensitivity

< 45.7 ng/ml

Range 0/100 - 10000 ng/ml
Assay time Sample acylation 90 min, ELISA overnight
Species reactivity Reacts with all species
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Product overview

Product name

L-Kynurenine ELISA kit

Description Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant. For reseach use only.
Format 96-well plate
Samples urine, plasma, serum, cell culture supernatant
Minimal sample volume 20 µL
Cross specie-reactivity React with all species
Standard range 0/100 - 10000 ng/ml
Sensitivity

< 47.5 ng/ml

Specificity

No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid.

Assay time Sample acylation 90 min and ELISA overnight
Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for use, Material Safety Datasheet
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Key steps

Sample collection & storage

Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample precipitation (90 min)

ELISA L-Kynurenine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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References

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