Kynurenine/Tryptophan ratio ELISA in IDOi-treated mice

Kynurenine/Tryptophan ratio ELISA in LPS-treated mice

Kynurenine / Tryptophan in cell culture supernatants

Kynurenine - Tryptophan ELISA standard curves

Kynurenine/Tryptophan ratio ELISA in IDOi-treated mice

Plasma samples were collected from MCA205 tumor-bearing mice treated with either vehicle or IDO inhibitor (GDC-0919) and determination of kynurenine to tryptophan ratio (KTR)

Kynurenine/Tryptophan ratio ELISA pack

Ref: ISE-2227

0 Reviews Ask ISE-2227 Product Overview Datasheet

The Kynurenine/Tryptophan ratio ELISA pack enables quantitative measurements of tryptophan catabolism along the kynurenine pathway in large numbers of samples. The two kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 20µl, they permit the performance of longitudinal studies in mice.

Sample type	Plasma, Serum, Cell culture supernatant, (Urine)
Kit capacity	2 x 96 tests
Sensitivity	LoD KYN: < 47.5 ng/ml LoD TRP: < 1.2µg/ml
Range	KYN: 0/100 - 10000 ng/ml TRP: 2.5 - 250 µg/ml
Species reactivity	Reacts with all species
References	Citations in literature

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Product information	Key steps	References	Technical support	Reviews

Product overview

Product name	Kynurenine/Tryptophan ratio ELISA pack
Description	Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine to L-Tryptophan ratio in urine, plasma and serum samples.
Labels	CE
Format	2 x 96-well plate
Samples	Plasma, serum, cell culture supernatant, (Urine)
Minimum sample volume	20 µL
Cross specie-reactivity	React with all species
Standard range	KYN: 0/100 - 10000 ng/ml TRP: 2.5 - 250 µg/ml
Sensitivity	LoD KYN: < 47.5 ng/ml LoD TRP: < 1.2µg/ml
Specificity	No significant cross-reactivity was observed with L-Tryptophan and L-Kynurenine analogs. See product pages for L-Kynurenine ELISA and L-Tryptophan ELISA.
Storage	Store at 2-8°C for to 6 months.
Datasheets	Instructions for use - TRP kit, Instructions for use -KYN kit

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Key steps

Sample collection & storage	Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)
Sample preparation	KYN: Sample acylation (90 min) TRP: Sample precipitation (15 min) and derivatization (2h)
ELISA	Antisera overnight incubation, revelation and read steps (1h).
Detailed protocol	Instructions for use - TRP kit, Instructions for use - KYN kit

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References

Product citations :

MacDowell et al. Paliperidone reversion of maternal immune activation-induced changes on brain serotonin and kynurenine pathways. Frontiers in Pharmacology. 2021 May 13

Baumgartner et al. Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans. Journal of Internal Medicine. 2021 Jan 289.

Müller-Thomas et al. Prognostic value of indoleamine 2,3 dioxygenase in patients with higher-risk myelodysplastic syndromes treated with azacytidine. British Journal of Haematology. 2020 Aug 190.

Adu-Gyamfi et al. Diagnostic accuracy of plasma kynurenine/tryptophan ratio, measured by enzyme-linked immunosorbent assay, for pulmonary tuberculosis. International Journal of Infectious Diseases. 2020 Aug 12.

Agullo-Ortuno et al. Blood Predictive Biomarkers for Patients With Nonesmall-cell Lung Cancer Associated With Clinical Response to Nivolumab. Clin Lung Cancer. 2020 Jan

Bekki et al. Serum kynurenine levels are a novel biomarker to predict the prognosis of patients with hepatocellular carcinoma. PLoS One 2020 Oct 21.

Nafia et al. IDO Targeting in Sarcoma: Biological and Clinical Implications. Front. Immunol. 2020 March 05

Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.

Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.

Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3

Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4

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Technical Support Submit a question

Asked
2015-07-14

Dear Sir, Has this ELISA kit been used and validated in humans? I see it is for all species, but would like to know any information on the use in humans. Thank you. Jin Hyong Jin Cho, MD, PhD

Answered
2015-07-14

Dear Dr Jin Hyong Cho, Many thanks for your interest. The IS-2227 pack is made of 2 kits : the Kynurenine ELISA kit BA-E-2200 and the Tryptophan ELISA kit BA-E-2700. The Tryptophan ELISA kit is an in vitro diagnostics product, intended for clinical use. The Kynurenine ELISA kit is for research use only. However, it has been used to analyze human samples (see cross-validation data with GC-MS on product page). Given that amino acids and their metabolites are preserved across species, the two kits can suit samples from any origin. I hope this helps. With best regards, Alban

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