Kynurenine/Tryptophan ratio ELISA in LPS-treated mice

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Kynurenine/Tryptophan ratio ELISA in LPS-treated mice

C57BL/6 mice were exposed to 10mg/kg of LPS or with PBS for 24 hours. Mice (n=5/experimental group) were bleeded at the tail vein using EDTA-Monovette®. Kynurenine and Tryptophan were quantified using ELISA kits. As an inducer of inflammatory response, LPS triggered a significant increase in plasmatic KTR level.

Kynurenine/Tryptophan ratio ELISA pack

Ref: ISE-2227

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The Kynurenine/Tryptophan ratio ELISA pack enables quantitative measurements of tryptophan catabolism along the kynurenine pathway in large numbers of samples. The two kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 20µl, they permit the performance of longitudinal studies in mice.

Sample type	Plasma, Serum, Cell culture supernatant, (Urine)
Kit capacity	2 x 96 tests
Sensitivity	LoD KYN: < 47.5 ng/ml LoD TRP: < 1.2µg/ml
Range	KYN: 0/100 - 10000 ng/ml TRP: 2.5 - 250 µg/ml
Species reactivity	Reacts with all species
Citations	4

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Product information	Key steps	References	Technical support	Reviews

Product overview

Product name	Kynurenine/Tryptophan ratio ELISA pack
Description	Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine to L-Tryptophan ratio in urine, plasma and serum samples.
Labels	CE
Format	2 x 96-well plate
Samples	Plasma, serum, cell culture supernatant, (Urine)
Minimum sample volume	20 µL
Cross specie-reactivity	React with all species
Standard range	KYN: 0/100 - 10000 ng/ml TRP: 2.5 - 250 µg/ml
Sensitivity	LoD KYN: < 47.5 ng/ml LoD TRP: < 1.2µg/ml
Specificity	No significant cross-reactivity was observed with L-Tryptophan and L-Kynurenine analogs. See product pages for L-Kynurenine ELISA and L-Tryptophan ELISA.
Storage	Store at 2-8°C for to 6 months.
Datasheets	Instructions for use - TRP kit, Instructions for use -KYN kit

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Key steps

Sample collection & storage	Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)
Sample preparation	KYN: Sample acylation (90 min) TRP: Sample precipitation (15 min) and derivatization (2h)
ELISA	Antisera overnight incubation, revelation and read steps (1h).
Detailed protocol	Instructions for use - TRP kit, Instructions for use - KYN kit

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References

Product citations :

Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.

Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.

Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3

Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4

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Technical Support Submit a question

Asked
2015-07-14

Dear Sir, Has this ELISA kit been used and validated in humans? I see it is for all species, but would like to know any information on the use in humans. Thank you. Jin Hyong Jin Cho, MD, PhD

Answered
2015-07-14

Dear Dr Jin Hyong Cho, Many thanks for your interest. The IS-2227 pack is made of 2 kits : the Kynurenine ELISA kit BA-E-2200 and the Tryptophan ELISA kit BA-E-2700. The Tryptophan ELISA kit is an in vitro diagnostics product, intended for clinical use. The Kynurenine ELISA kit is for research use only. However, it has been used to analyze human samples (see cross-validation data with GC-MS on product page). Given that amino acids and their metabolites are preserved across species, the two kits can suit samples from any origin. I hope this helps. With best regards, Alban

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