L-Kynurenine accumulation in human colorectal tumor by IHC L-Kynurenine accumulation in human colorectal tumor by IHC L-Kynurenine detection in mouse spleen tissue by IF Affinity & specificity of L-Kynurenine polyclonal antibody L-Kynurenine (KYN)

L-Kynurenine accumulation in human colorectal tumor by IHC

Immunohistochemical analysis reveals cytoplasmic accumulation of L-Kynurenine in tumour cells in human colorectal cancer tissue. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval, and overnight incubation with primary anti-KYN polyclonal antibody (1/500 dilution). A polymer-conjugated secondary Ab was added and immunostaining was revealed using DAB.

L-Kynurenine polyclonal antibody

Ref: IS1012

 

Validated for IHC in human tumor tissues, the anti-L-Kynurenine (Kyn) rabbit polyclonal antibody proved to work at 1/500 dilution on paraffin-embedded sections, a single vial thus catering for approximately 100 stainings.

 

Clonality Polyclonal
Host Rabbit
Validated applications IHC
Specie reactivity Reacts with all species
References Not yet cited to our knowledge. 
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Product information Protocols References Technical support Reviews

Product overview

 

Product name L-Kynurenine polyclonal antibody
Synonyms

KYN polyclonal antibody

(S)-Kynurenine polyclonal antibody

L-2-Amino-4-(2-aminophenyl)-4-oxobutanoic acid antibody

Kynurenine polyclonal antibody

3-Anthraniloyl-L-alanine polyclonal antibody

Immunogen Conjugated L-Kynurenine
Specificity When tested in competitive ELISA, the anti-L-Kynurenine polyclonal antibody did not show any significant cross reactivity with competitors including anthranilic acid, 3-OH-Kynurenine conjugate and Tryptophan.
Lot number

140401 - Use before April 2021

 

Storage

 

Form

Liquid

Purity Purified anti-serum
Storage Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
Material safety datasheet

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Protocols

Immunohistochemistry (IHC)

Dilute at 1:100-1:1000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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