Cross-validation of L-Serine ELISA and LC/MS data in human plasma samples Cross-validation of L-Serine ELISA and LC/MS data in human plasma samples Typical standard curve of Serine ELISA # IS-I-1200 : L-Serine (Ser) ELISA kit

Cross-validation of L-Serine ELISA and LC/MS data in human plasma samples

Ser was quantified in human plasma samples from 40 healthy subjects using IS-I-1200 ELISA kit or by LC/MS. Correlation study showed R2=0.957, thereby confirming the accuracy of the immunoassay.

L-Serine ELISA kit

Ref: IS-I1200

L-Serine (L-Ser) is a non-essential amino acid which becomes conditionally essential - in certain circumstances - particularly when it cannot be synthesized in a sufficient amount to meet the cellular demands. L-Ser may be derived from different sources including dietary intake, the glycolytic intermediate 3-phosphoglycerate, glycine as well as protein and phospholipid degradation. It’s worth noting that enzymes involved in L-Ser biosynthesis are upregulated in highly proliferating cells, including cancer cells, to limit L-Ser over-consumption. Besides its essential role in cell proliferation, L-Ser is important for brain development and functions which are especially sustained by i) altered L-Ser levels in psychiatric disorders and ii) occurrence of severe neurological abnormalities in patients with defects in L-Ser biosynthesis.

 

Sample type
Plasma, Serum, Cell culture supernatant
Capacity

96 tests

Sensitivity 2.4µM
Range 3.8 - 625µM
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species

 

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Product overview

Product name L-Serine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Serine (Ser) in plasma and serum samples. For research use only.
Format 96-well plate
Samples Plasma, Serum, Cell supernatant
Minimal sample volume 10 µL
Species reactivity React with all species
Standard range 3.8 - 625 µM
Sensitivity

2.4µM

Specificity

No significant cross-reactivity was observed with Glycine, L-Threonine, L-Cysteine and D-Serine.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  - Do not use for diagnostic

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Key steps

Sample collection & storage

EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Serine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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