Cross-validation of L-Serine ELISA and LC/MS data in human plasma samples
Ser was quantified in human plasma samples from 40 healthy subjects using IS-I-1200 ELISA kit or by LC/MS. Correlation study showed R2=0.957, thereby confirming the accuracy of the immunoassay.
L-Serine ELISA kitRef: IS-I1200
L-Serine (L-Ser) is a non-essential amino acid which becomes conditionally essential - in certain circumstances - particularly when it cannot be synthesized in a sufficient amount to meet the cellular demands. L-Ser may be derived from different sources including dietary intake, the glycolytic intermediate 3-phosphoglycerate, glycine as well as protein and phospholipid degradation. It’s worth noting that enzymes involved in L-Ser biosynthesis are upregulated in highly proliferating cells, including cancer cells, to limit L-Ser over-consumption. Besides its essential role in cell proliferation, L-Ser is important for brain development and functions which are especially sustained by i) altered L-Ser levels in psychiatric disorders and ii) occurrence of severe neurological abnormalities in patients with defects in L-Ser biosynthesis.
||Plasma, Serum, Cell culture supernatant
|Range||3.8 - 625µM|
|Assay time||Sample preparation 3h, ELISA overnight|
|Species reactivity||Reacts with all species|
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|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
|Product name||L-Serine ELISA kit|
|Description||Competitive ELISA kit for the quantitative measurement of L-Serine (Ser) in plasma and serum samples. For research use only.
|Samples||Plasma, Serum, Cell supernatant|
|Minimal sample volume||10 µL
|Species reactivity||React with all species|
|Standard range||3.8 - 625 µM|
No significant cross-reactivity was observed with Glycine, L-Threonine, L-Cysteine and D-Serine.
|Assay time||Sample preparation 3h and ELISA overnight|
|Storage||Store at 2-8°C for up to 6 months.|
|Datasheets||Instructions for use, Material Safety Datasheet|
For research use only - Do not use for diagnosticTop
|Sample collection & storage||
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation (3 hours)
|ELISA||L-Serine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
Selected articles on Serine:
- Mattaini et al., The importance of serine metabolism in cancer, J Cell Biol, 2016
- Tabatabaie et al., L-serine synthesis in the central nervous system: a review on serine deficiency disorders, Mol Genet Metab, 2010
- Koning et al., L-serine in disease and development, Biochemical Journal, 2003