Tryptophan immunostaining in the CNS of embryonic mouse Tryptophan immunostaining in the CNS of embryonic mouse Tryptophan detection in the crayfish brain Affinity & specificity of anti- L-Tryptophan antibody Amino acid L-Tryptophan

Tryptophan immunostaining in the CNS of embryonic mouse

Using STAINperfect immunostaining kit A, Tryptophan was detected in the hindbrain of embryonic mouse E15.5. Staining was obtained following the protocol optimized for whole mount using IS011 mouse monoclonal antibody targeting L-Tryptophan. Fluorescent conjugated secondary antibodies were then used and images captured by confocal microscopy at high magnification.

L-Tryptophan antibody (mouse monoclonal)

Ref: IS011

 

The anti-Tryptophan antibody IS011 is a mouse monoclonal antibody optimized to ensure high affinity and specificity. Combined with the STAINperfect immunostaining kit A, this antibody allows direct L-Tryptophan visualization in whole mount, cell culture and tissue sections.

Clonality Monoclonal antibody (clone 4H11-A11)
Host Mouse
Species reactivity Reacts with all species
Tested samples whole mount, cell culture, tissue sections
Staining procedure STAINperfect immunostaining kit A
Format 50µL (approx. 40 tissue sections)
370
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Product information Protocols References Technical support Reviews

 

Product overview

 


 

Product name

L-Tryptophan antibody

Synonyms

Anti-L-Trp antibody

(S)-2-Amino-3-(3-indolyl)propionic acid antibody

L-alpha-Amino-3-indolepropionic acid antibody

(S)-2-Amino-3-(3-indolyl)propanoic acid antibody

L-alpha-Amino-3-indolepropanoic acid antibody

Immunogen Conjugated L-Tryptophan
Isotype IgG1 k chain
Specificity When tested in competitive ELISA, the anti-conjugated L-Tryptophan antibody did not show any significant cross reactivity with Tryptophan analogs, including Tryptamine
Volume 50µL
Lot number

140201

 

 

Storage

 


 

Form

Liquid

Purity Purified IgG
Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

IF - Cell cultures, Whole mounts, Tissue sections

Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol for dopamine staining
Protocols-at-a-glance

 

 

STAINperfect neurotransmitters instructions for use

STAINperfect neurotransmitter staining protocol cell cultures STAINperfect neurotransmitter staining protocol whole mounts STAINperfect neurotransmitter staining protocol tissue sections
Complete 
Instructions for Use
Protocol-at-a-glance 
for cell cultures
Protocol-at-a-glance 
for whole mounts
Protocol-at-a-glance 
for tissue sections


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