Histamine ELISA kit
Ultra-Sensitive – Any sample

Ref: BA-E-5800R
NeuromediatorsHistamineFood safetyResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

This research Histamine ELISA kit enables the ultra-sensitive measurement of histamine in any biological sample: tissue homogeneates, cell culture supernatants, body fluids, etc. With a minimal sample volume as low as 10µl, this histamine immunoassay allows longitudinal studies in mice and rat models.

Sample type10µl
Samples/kit40 samples in duplicate
Sensitivity0.2ng/ml
Range0.5 – 50ng/ml
Assay timeSample acylation (1h30) and ELISA overnight
ReactivityReacts with all species
CitationsCited in 5 papers 
Size

1 x 96 wells

506.00

97 in stock

Quantity
Total

506.00

Worldwide shipping

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Product overview

Product nameHistamine ELISA kit – Any sample – Research
DescriptionEnzyme immunoassay (ELISA) for the quantitative determination of histamine levels in biological samples. Research use only
Format96-well plate
Samples10µl, any sample
ReactivityReacts with all species
Standard range0 / 0.5 – 50ng/ml
Sensitivity

0.2 ng/ml

SpecificityNo significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin
Assay time1h30 sample acetylation + ELISA overnight
StorageStore at 2-8°C for up to 6 months
DatasheetsInstructions for use, Material safety datasheet

Sample preparationSample predilution and acylation (1h30)
ELISAHistamine antiserum incubation (3H or overnight), revelation and read steps (1h30)
Detailed protocolDownload instructions for use

Product citation

  • Establishment and Characterization of Mild Atopic Dermatitis in the DNCB-Induced Mouse Model
    Check the article
    Authors : Riedl et al.
    2023-08
  • Ganglioside GM3 deficiency enhances mast cell sensitivity
    Check the article
    Authors : Komuro et al.
    2023-04
  • Streptococcal sagA activates a proinflammatory response in mast cells by a sublytic mechanism
    Check the article
    Authors : Von Beek et al., Cellular Microbiology
    2019-07
  • Decreased viability and proliferation of CHANG conjunctival epithelial cells after contact with ultraviolet light-irradiated pollen
    Check the article
    Authors : Heidinger et al., Cutaneous & Ocular Toxicology
    2017-12
  • Fermented fish oil suppresses T helper 1/2 cell response in a mouse model of atopic dermatitis via generation of CD4+CD25+Foxp3+ T cells
    Check the article
    Authors : Han et al., BMC Immunology
    2012-08
  • Smoking influences salivary histamine levels in periodontal disease
    Check the article
    Authors : Bertl et al., Oral Diseases
    2012-01
  • Mesenteric lymph node transcriptome profiles in BALB/c mice sensitized to three common food allergens
    Check the article
    Authors : Husain et al., BMC Genomics
    2011-01
  • Multiple T cell epitope peptides suppress allergic responses in an egg allergy mouse model by the elicitation of forkhead box transcription factor 3- and transforming growth factor-beta-associated mechanisms
    Check the article
    Authors : Yang et al., Clinical & Experimental Allergy
    2010-01
  • Immunomodulatory effects of egg white enzymatic hydrolysates containing immunodominant epitopes in a BALB/c mouse model of egg allergy
    Check the article
    Authors : Yang et al., Journal of agricultural & food chemistry
    2009-03

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