Histamine release kit
Whole blood samples

Ref: BA-E-1100
Diagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISANeuromediatorsHistamine
DatasheetProtocolMSDS

Intended for in vitro diagnostics & research, this add-on kit to the BA-E-1000 histamine ELISA kit enables the measurement of histamine release in heparinized whole blood samples, allowing for instance to determine the sensitivity of basophilic granulocytes to specific allergens.

Sample type500µl whole blood
Samples/kit40 samples in duplicate
Sensitivity0.2ng/ml
Range0.5 – 50ng/ml
Assay timeSample preparation (1h30)
ReactivityReacts with all species
ReferencesCited in 2 papers
Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

86.00

Clear
Quantity
Total

86.00

Worldwide shipping

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Product overview

Product nameHistamine release kit – Whole blood samples
DescriptionSupplementary kit to in vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine release in whole blood samples
LabelsIVD, CE
Format96-well plate
Samples500µl whole blood
ReactivityReacts with all species
Standard range0 / 0.5 – 50ng/ml
Sensitivity

0.2ng/ml

SpecificityNo significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin
Assay time1h30 sample preparation + ELISA overnight
StorageStore at 2-8°C for up to 6 months
DatasheetsInstructions for useMaterial safety datasheet

 

Sample collection & storage24-hours heparinized whole blood stored at 20-25°C
Sample preparationSample acylation (1h30)
ELISAHistamine antiserum incubation (3H or overnight), revelation and read steps (1h)
Detailed protocolDownload instructions for use

Product citation

  • Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets
    Check the article
    Authors : Chirumbolo et al., Clinical & Molecular Allergy
    2010-09
  • H2-receptor-mediated vasodilation contributes to postexercise hypotension
    Check the article
    Authors : McCord et al., Journal of Applied Physiology
    2005-09

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