Histamine release kit
Whole blood samples

Ref: BA-E-1100
Research ELISA kits (RUO)ELISANeuromediatorsHistamineDiagnostic ELISA kits (IVD)
DatasheetProtocolMSDS

Intended for in vitro diagnostics & research, this add-on kit to the BA-E-1000 histamine ELISA kit enables the measurement of histamine release in heparinized whole blood samples, allowing for instance to determine the sensitivity of basophilic granulocytes to specific allergens.

Sample type 500µl whole blood
Samples/kit 40 samples in duplicate
Sensitivity 0.2ng/ml
Range 0.5 – 50ng/ml
Assay time Sample preparation (1h30)
Reactivity Reacts with all species
References Cited in 2 papers
Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

86.00

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Quantity
Total

86.00

Worldwide shipping

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Product overview

Product name Histamine release kit – Whole blood samples
Description Supplementary kit to in vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine release in whole blood samples
Labels IVD, CE
Format 96-well plate
Samples 500µl whole blood
Reactivity Reacts with all species
Standard range 0 / 0.5 – 50ng/ml
Sensitivity

0.2ng/ml

Specificity No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin
Assay time 1h30 sample preparation + ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial safety datasheet

 

Sample collection & storage 24-hours heparinized whole blood stored at 20-25°C
Sample preparation Sample acylation (1h30)
ELISA Histamine antiserum incubation (3H or overnight), revelation and read steps (1h)
Detailed protocol Download instructions for use

Product citation

  • Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets
    Check the article
    Authors : Chirumbolo et al., Clinical & Molecular Allergy
    2010-09
  • H2-receptor-mediated vasodilation contributes to postexercise hypotension
    Check the article
    Authors : McCord et al., Journal of Applied Physiology
    2005-09

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