Antibodies & immunoassays for small molecules Serotonin rabbit pAb – IS1007
Ref: IS1007-sp
NeuromediatorsTryptophan metabolismPolyclonal AntibodiesICCIFIHC
+Presence of serotonin in neurons of crayfish brain
High magnification of serotoninergic neurons in the brain of a crayfish. This staining shows serotonin presence in soma containing vesicules and projections. Tissue was processed following whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibody was used and picture was acquired by confocal imaging.
DatasheetMSDS
The anti-Serotonin antibody IS1007 is a rabbit polyclonal antibody with high affinity and specificity. Using with the STAINperfect immunostaining kit A, our antibody labeled directly 5-HT in whole mounts, cell culture and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 25µL (approx. 20 tissue sections) |
Product overview
| Product name | Serotonin antibody (5-HT rabbit polyclonal Ab) |
| Synonyms | Anti-5-HT antibody
Anti-5-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-Methoxytryptamine |
| Volume | 25µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Serotonin staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
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Selected papers about serotonin:
- Gershon MD. 5-Hydroxytryptamine (serotonin) in the gastrointestinal tract. Curr Opin Endocrinol Diabetes Obes. 2013 Feb;20(1):14-21. doi: 10.1097/MED.0b013e32835bc703.
- Baganz NL, Blakely RD. A dialogue between the immune system and brain, spoken in the language of serotonin. ACS Chem Neurosci. 2013 Jan 16;4(1):48-63. doi: 10.1021/cn300186b. Epub 2012 Dec 7.
- Bortolato M et al. The role of the serotonergic system at the interface of aggression and suicide. Neuroscience. 2013 Apr 16;236:160-85.
- Kiyasova V et al. A genetically defined morphologically and functionally unique subset of 5-HT neurons in the mouse raphe nuclei. J Neurosci. 2011 Feb 23;31(8):2756-68. doi: 10.1523/JNEUROSCI.4080-10.2011.
