Antibodies & immunoassays for small molecules Xanthurenic acid Antibody – Mouse Monoclonal
Ref: IS009
Kynurenine pathwayMonoclonal AntibodiesICCIFIHC
+Xanthurenic acid detection by IF in human midbrain
Xanthurenic acid visualization by immunohistofluorescence in human caudate putamen. Staining highlights cytoplasmic accumulation of Xanthurenic acid in brain cells. Paraffin-embedded brain tissue section was subjected to pH=9 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/500). After incubation with Alexa-488 conjugated secondary Ab, epifluorescence microscopy (100X) was used to capture the staining.
DatasheetMSDS
IS009 was the first anti-Xanthurenic acid antibody made available to the scientific community, back in 2014. This mouse mAb was validated for IHC and IF in human midbrain tissues, highlighting cytoplasmic presence of xanthurenic acid.
| Clonality | Monoclonal antibody (clone 6C12-A12) |
| Host | Mouse |
| Valided applications | IHC / IF |
| Reactivity | Reacts with all species |
| Format | 50µL |
Product overview
| Product name | Xanthurenic acid antibody |
| Synonyms | Anti- Xanthurenic acid antibody
8-Hydroxy-4-oxo-1H-quinoline-2-carboxylic acid antibody Xanthuric acid antibody Xanthurenate antibody 8-Hydroxykynurenic acid antibody |
| Immunogen | Conjugated Xanthurenic acid |
| Clone | clone 6C12-A12 |
| Specificity | When tested in competitive ELISA, the anti-conjugated Xanthurenic acid antibody did not show any significant cross-reactivity with competitors Kynurenic acid and Quinaldic acid conujugates |
Storage
| Form | Liquid |
| Purity | Purified IgG |
| Concentration | 0,5mg/ml |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| Immunohistochemistry (IHC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=9) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) | Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
Selected publications on Xanthurenic acid
- Neale SA, Copeland CS, Salt TE.Effect of VGLUT inhibitors on glutamatergic synaptic transmission in the rodent hippocampus and prefrontal cortex.Neurochem Int. 2013 Oct 10. pii: S0197-0186(13)00250-7. doi: 10.1016/j.neuint.2013.10.001. [Epub ahead of print]
- Neale SA, Copeland CS, Uebele VN, Thomson FJ, Salt TE. Modulation of hippocampal synaptic transmission by the kynurenine pathway member xanthurenic acid and other VGLUT inhibitors. Neuropsychopharmacology. 2013 May;38(6):1060-7. doi: 10.1038/npp.2013.4. Epub 2013 Jan 7.
- Taleb O, Maammar M, Brumaru D, Bourguignon JJ, Schmitt M, Klein C, Kemmel V, Maitre M, Mensah-Nyagan AG.Xanthurenic acid binds to neuronal G-protein-coupled receptors that secondarily activate cationic channels in the cell line NCB-20.PLoS One. 2012;7(11):e48553. doi: 10.1371/journal.pone.0048553. Epub 2012 Nov 6.
- Copeland CS1, Neale SA, Salt TE.Actions of Xanthurenic acid, a putative endogenous Group II metabotropic glutamate receptor agonist, on sensory transmission in the thalamus.Neuropharmacology. 2013 Mar;66:133-42. doi: 10.1016/j.neuropharm.2012.03.009. Epub 2012 Apr 2.
