L-Tryptophan ELISA kit
Any sample I High Sensitivity

Ref: BA-E-2700
Tryptophan metabolismAmino acidsDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
Tryptophan ELISA kit high sensitivity+
Tryptophan ELISA kit
Tryptophan ELISA kit high sensitivity
Murine CT26 tumor cell line was stably transfected with either empty vector (Mock) or vector containing cDNA encoding for indoleamine 2,3 dioxygenase (IDO1). Cells were cultured for 48 hours and Tryptophan was measured using the Tryptophan ELISA kit. As expected, Tryptophan level was significantly lower in IDO1-proficient CT26 cells than in mock or parental (WT) CT26 cell lines.
Example of standard curve obtained with the L-Tryptophan ELISA Kit. In this competitive ELISA, optical density is invertly correlated with Tryptophan levels. Only serves as example - do not use for calculation.
DatasheetMSDSIVD ProtocolRUO Protocol

This Tryptophan ELISA kit enables the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Intended for in vitro diagnotics and research applications, the test comes in 96-well plate format, allowing the screening of large experimental series within short lead times.

Sample typeBiological fluids (urine, plasma, serum, …) and cell culture supernatant
Capacity96 tests
Sensitivity< 1.2µg/ml
Range2.5 – 250µg/ml
Assay time2h30 sample preparation and ELISA overnight
ReactivityReacts with all species
ReferencesCited in >40 papers

 

Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

481.10$

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481.10$

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Product overview

Product nameTryptophan ELISA kit
DescriptionIn vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, …) and cell culture supernatants
LabelsIVD, CE
Format96-well plate
Samplesurine, plasma, serum, cell culture supernatant
Sample volume20µL
ReactivityReacts with all species
Standard range2.5 – 250µg/ml
Sensitivity

< 1.2µg/ml

SpecificityNo significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine
Assay timeSample preparation 2.5 hours and ELISA overnight
StorageStore at 2-8°C for to 6 months
DatasheetsInstructions for use , Material safety datasheet

 

Sample collection & storageUrine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)

Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples

Sample preparationSample precipitation (15 min) and derivatization (2h15)
ELISATryptophan antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolIVD: Download instructions for use

RUO: Download instructions for use

Product citation

  • Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy
    Check the article
    Authors : Wang et al., Nature Communications
    2023-11
  • Dietary Marine Hydrolysate Improves Memory Performance and Social Behavior through Gut Microbiota Remodeling during Aging
    Check the article
    Authors : Mougin et al., Foods
    2023-11
  • An open label randomized controlled trial of atorvastatin versus aspirin in elite controllers and antiretroviral-treated people with HIV
    Check the article
    Authors : Mystakeli et al., AIDS
    2023-10
  • Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress
    Check article
    Authors : Letenneur et al., Nutrients
    2023-10
  • Hypomethylation-induced regulatory programs in T cells unveiled by transcriptomic analyses
    Check the article
    Authors : Lysandrou et al., Frontiers in Immunology
    2023-09
  • Pathogenetic associations of anti-ribosomal P protein antibody titres and their subclasses in patients with systemic lupus erythematosus
    Check the article 
    Authors : Kaneko et al., Rheumatology
    2023-08
  • Tryptophan Modulation in Cancer-Associated Cachexia
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    Authors : Agullo-Ortuno et al., International Journal of Molecular Sciences
    2023-08
  • Intake of Lactiplantibacillus plantarum HEAL9 Improves Cognition in Moderately Stressed Subjects: A Randomized Controlled Study
    Check the article
    Authors : Onning et al., Nutrients
    2023-08
  • Effects of Supplementation with the Standardized Extract of Saffron (affron®) on the Kynurenine Pathway and Melatonin Synthesis in Rats
    Check the article
    Authors : Munoz et al., Antioxydants
    2023-07
  • Advances in kynurenine analysis
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    Authors : Mrstna et al., Clinica Chimica Acta
    2023-07
  • Involvement of the kynurenine pathway in breast cancer: updates on clinical research and trials
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    Authors : Girithar et al., British Journal of Cancer
    2023-04
  • Tryptophan degradation enzymes expression in the placenta and the Kynurenine/Tryptophan ratio in maternal plasma after elective cesarean section
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    Authors : Silvano et al., Journal of Reproductive Immunology
    2023-02
  • Citrulline and kynurenine to tryptophan ratio: potential EED (environmental enteric dysfunction) biomarkers in acute watery diarrhea among children in Bangladesh
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    Authors : Das et al., Scientific Reports
    2023-01
  • Tryptophan degradation enzymes and Angiotensin (1−7) expression in human placenta
    Check the article
    Authors : Silvano et al., Journal of Reproductive Immunology
    2022-09
  • IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma
    Check the article
    Authors : Struckmeier et al., Journal of Cancer Reasearch & Clinical Oncology
    2022-08
  • Nociceptor-derived Reg3g prevents endotoxic death by targeting kynurenine pathway in microglia
    Check the article
    Authors : Sugisawa et al., Cell reports
    2022-03
  • Tryptophan Metabolism Is Associated with BMI and Adipose Tissue Mass and Linked to Metabolic Disease in Pediatric Obesity
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    Authors : Lischka et al, Nutrients
    2022-01
  • Apathy is not associated with a panel of biomarkers in older adults with HIV disease
    Check the article
    Authors : Wood et al., Journal of psychosomatic research
    2021-11
  • Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway
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    Authors : Kim et al., Oncoimmunology
    2021-11
  • Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma
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    Authors : Pospíšková et al., Pteridines
    2021-11
  • The Role of Indoleamine 2,3-Dioxygenase in Renal Tubular Epithelial Cells Senescence under Anoxia or Reoxygenation
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    Authors : Eleftheriadis et al., Biomolecules
    2021-10
  • Plasma Kynurenine-to- Tryptophan Ratio, a Highly Sensitive Blood-Based Diagnostic Tool for Tuberculosis in Pregnant Women Living With Human Immunodeficiency Virus (HIV)
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    Authors : Adu-Gyamfi et al., Clinical infectious Diseases
    2021-10
  • The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)
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    Authors : Francikowski et al., Scientific reports
    2021-10
  • Abnormal Tryptophan Metabolism in HIV and Mycobacterium tuberculosis Infection
    Check the article
    Authors : Wang et al., Frontiers in microbiology
    2021-06
  • Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza
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    Authors : Jung Cho et al., European Respiratory Journal
    2021-05
  • Role of indoleamine 2,3-dioxygenase in ischemia-reperfusion injury of renal tubular epithelial cells
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    Authors : Eleftheriadis et al., Molecular Medicine Reports
    2021-04
  • Mood Disorder in Systemic Lupus Erythematosus Induced by Antiribosomal P Protein Antibodies Associated with Decreased Serum and Brain Tryptophan
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    Authors : Cho et al., journal of Immunology
    2021-03
  • Early-Life Stress Modulates Gut Microbiota and Peripheral and Central Inflammation in a Sex-Dependent Manner
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    Authors : Park et al., International journal of molecular sciences
    2021-02
  • Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans
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    Authors : Baumgartner et al., Journal of internal medicine
    2020-08
  • Diagnostic accuracy of plasma kynurenine/tryptophan ratio, measured by enzyme-linked immunosorbent assay, for pulmonary tuberculosis
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    Authors : Adu-Gyamfi et al., International journal of infectious diseases
    2020-08
  • Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients
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    Authors : Masachika et al., Clinical neuroscience
    2020-06
  • Prognostic value of indoleamine 2,3 dioxygenase in patients with higher-risk myelodysplastic syndromes treated with azacytidine
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    Authors : Müller-Thomas et al., British journal of haematology
    2020-04
  • Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8 + T-Cell-Dependent Tumor Activity
    Check the article
    Authors : Watanabe et al., Clinical Cancer Research
    2019-11
  • Blood Predictive Biomarkers for Patients With Non-small-cell Lung Cancer Associated With Clinical Response to Nivo_lumab
    Check the article
    Authors : Agullo-Ortuno et al., Clinical lung cancer
    2019-08
  • Traumatic injury leads to inflammation and altered tryptophan metabolism in the juvenile rabbit brain
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    Authors : Zhang et al., Journal of Neurotrauma
    2018-09
  • Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
    Check the article
    Authors : Walker et al., Molecular Psychiatry
    2018-07
  • Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
    Check the article
    Authors : Martin-Hernandez et al., Molecular Neurobiology
    2018-05
  • Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
    Check the article
    Authors : Ziklo et al., Frontiers in cellular and infection microbiology
    2018-01
  • In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis
    Check the article
    Authors : Gautam et al., Proceedings of the National Academy of Sciences of the USA
    2018-01
  • Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
    Check the article
    Authors : Toulmonde et al., JAMA oncology
    2018-01
  • In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp
    Check the article
    Authors : Ziklo et al. BMC Microbiology
    2016-12

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