L-Tryptophan ELISA Kit
Essential amino acid L-Tryptophan is either converted into Serotonin in the gut or brain by tryptophan hydroxylase (TPH), or metabolized by various cell types to form L-Kynurenine through enzymes indoleamine 2,3-dioxygenase (IDO1/2) or tryptophan 2,3-dioxygenase (TDO2). Abnormal tryptophan metabolism is found in a wide range of pathologies, including metabolic and auto-immune diseases, viral infections, cancer, as well as neurodegenerative and mood disorders.
L-Tryptophan ELISA kit
Ref: BA-E-2700
This Tryptophan ELISA kit enables the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Intended for in vitro diagnotics and research applications, the test comes in 96-well plate format, allowing the screening of large experimental series within short lead times.
| Sample type |
Biological fluids (urine, plasma, serum, ...) and cell culture supernatant. |
| Capacity | 96 tests |
| Sensitivity |
< 1.2µg/ml |
| Range | 2.5 - 250 µg/ml |
| Assay time | 2h30 sample preparation and ELISA overnight |
| Species reactivity | Reacts with all species |
| References | Citations in the litterature |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Tryptophan ELISA kit |
| Description | In vitro diagnostic enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, ...) and cell culture supernatants. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | urine, plasma, serum, cell culture supernatant |
| Sample volume | 20 µL |
| Cross species-reactivity | React with all species |
| Standard range | 2.5 - 250 µg/ml |
| Sensitivity |
< 1.2µg/ml |
| Specificity |
No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine. |
| Assay time | Sample preparation 2.5 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage |
Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months) |
| Sample preparation |
Sample precipitation (15 min) and derivatization (2h15) |
| ELISA | Tryptophan antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
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