Total and GABAergic neuronal networks in mature rat cortical cultures
Image field micrographs of mature (12 DIV-old) rat cortical cultures, illustrating total and GABAergic neuronal networks revealed by MAP2 (in red) and GABA (in green) immunostaining, respectively. GABA immunostaining obtained using whether our monoclonal (#IS039) or polyclonal GABA antibody (#IS1006) with the STAINperfect immunostaining kit A is similar thereby underlining GABAergic cell bodies and dense punctiform GABAergic neurites.
GABA antibody - Mouse monoclonal AbRef: IS039
Anti-GABA antibody (#IS039) is a mouse monoclonal antibody specifically selected by competitive ELISA for its affinity and specificity features. Used with the STAINperfect immunostaining kit A through appropriate protocols, our antibody directly labels GABA and allows to evidence, in the same way as with our validated reference rabbit polyclonal anti-GABA antibody (#IS1006), GABAergic systems – cell bodies and neuritic network, in whole mounts, cell culture and tissue sections.
|Clonality||Monoclonal antibody (clone 7F52B41B4)
|Species reactivity||Reacts with all species
|Applications||IHC / IF|
|Tested samples||whole mounts, cell culture, tissue sections|
|Staining procedure||STAINperfect immunostaining kit A|
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
GABA antibody - mouse mAb
Anti-g-Aminobutyric acid antibody
Anti-gamma-Aminobutyric acid antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and L.Glutamate.|
|Purity||Purified IgG1 (lambda)
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS
IF - Cell cultures, Whole mounts, Tissue sections
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for GABA staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
Antibody not yet cited. Submit an article and get a 10% discount.
Selected articles on GABA:
- Liu X, Wang Q, Haydar TF, Bordey A. Nonsynaptic GABA signaling in postnatal subventricular zone controls proliferation of GFAP-expressing progenitors. Nat Neurosci. 2005 Sep;8(9):1179-87. Epub 2005 Aug 14.
- Lawrence JJ. Cholinergic control of GABA release: emerging parallels between neocortex and hippocampus. Trends Neurosci. 2008 Jul;31(7):317-27. doi: 10.1016/j.tins.2008.03.008. Epub 2008 Jun 13.
- Baulac S, Huberfeld G, Gourfinkel-An I, Mitropoulou G, Beranger A, Prud'homme JF, Baulac M, Brice A, Bruzzone R, LeGuern E. First genetic evidence of GABA(A) receptor dysfunction in epilepsy: a mutation in the gamma2-subunit gene. Nat Genet. 2001 May;28(1):46-8.
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