Fast Urine 5-HIAA ELISA Fast Urine 5-HIAA ELISA 5-HIAA ELISA: Standard curve

Fast Urine 5-HIAA ELISA

5-HIAA (5-Hydroxyindoleacetic acid) is the main metabolite of serotonin. Urinary excretion of 5-HIAA is routinely used as a surrogate biomarker to diagnose and monitor carcinoid tumors. In the cerebrospinal fluid, low levels of 5-HIAA have been found to be associated with agressive behavior and suicide by violent means.

5-HIAA ELISA kit - Urine - Fast Track

Ref: BA-E-1900

 

This 5-hydroxyindoleacetic acid (5-HIAA) ELISA kit is tailored for the rapid measurement of 5-HIAA in urine samples (specific protocols for CSF and plasma are available). Intended for in vitro diagnostics, this fast-track quantitative immunoassay can be performed within 3 hours.

 

Sample type
50 µl urine
Samples/kit 40 samples in duplicate
Sensitivity

0.17 mg/l

Range 0 / 0.5 - 50 mg/l
Assay time 3h
Species reactivity Reacts with all species
Citations 2
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Product information Key steps References Technical support Reviews

Product overview

Product name

5-HIAA ELISA kit - Urine - Fast-Track

Description In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of 5-HIAA in urine samples.
Labels IVD, CE
Format 96-well plate
Samples 50 µl urine
Cross specie-reactivity React with all species
Standard range 0 / 0.5 - 50 mg/l
Sensitivity

0.17 mg/l

Specificity

No significant cross-reactivity was observed with 5-HIAA analogs such as Serotonin, 5-Hydroxy-DL-Tryptophan, Tryptamine, Melatonin, 5-Hydroxytryptamin, Vanillic mandelic acid and Homovanillic Acid.

Assay time 3 hours
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for use Material safety datasheet
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Key steps

Sample collection & storage

Spontaneous or 24-hour urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C.

Sample preparation

Sample predilution and methylation (30 min)

ELISA 5-HIAA antiserum incubation (1 hour), revelation and read steps (90 min).
Detailed protocol Download instructions for use
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