Sensitive 5-HT ELISA kit Sensitive 5-HT ELISA kit

Sensitive 5-HT ELISA kit

Serotonin (5-hydroxytryptamine) is a monoamine neurotransmitter derived from the essential amino acid L-tryptophan. Produced by enterochromaffin cells in the gut and serotonergic neurons in the brain, serotonin is stored and transported by platelets in the blood. It is then excreted in urine directly, or in the form of 5-HIAA, its main metabolite. Altered serotonin levels in serum, urine and platelets are associated with carcinoid tumors and CNS disorders such as autism, depression and suicidal schizophrenia.

Serotonin ELISA kit - Research

Ref: BA-E-5900

 

This Serotonin (5-HT) ELISA kit enables the ultra-sensitive measurement of 5-hydroxytryptamine in any biological sample. Intended for research use only, the assay features a lower limit of detection at 5 pg/ml and a minimal sample volume as low as 1µl.

 

Sample type
1 - 100 µl - any sample
Samples/kit 40 samples in duplicate
Sensitivity

5 pg/ml

Range 0 / 0.015 - 2.5 ng/ml
Assay time

30 min sample preparation, ELISA overnight

Species reactivity Reacts with all species
Citations Over 20
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Product information Key steps References Technical support Reviews

Product overview

Product name

Serotonin ELISA kit - Research

Description Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Serotonin in biological samples. Research use only.
Format 96-well plate
Samples 1 - 100 µl - any biological sample
Cross-species reactivity React with all species
Standard range 0 / 0.015 - 2.5 ng/ml
Sensitivity

5 pg/ml

Specificity

No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan.

Assay time 30 min + overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample preparation

Sample acylation (30min)

ELISA Serotonin antiserum incubation (overnight), revelation and read steps (1h).
Detailed protocol Download instructions for use
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References

 

 

 

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