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Histamine Antibody – Rabbit Polyclonal
Ref: IS1039NeuromediatorsHistaminePolyclonal AntibodiesICCIFIHC
+Immunofluorescence imaging of Histamine in mouse midbrain
Histamine was detected into whole mount mouse midbrain using STAINperfect immunostaining kit A. Staining was obtained following the protocol for whole mounts samples preparation combined with our IS1039 rabbit polyclonal anti-histamine antibody. After incubation with a fluorescent labeled secondary antibody, confocal imaging was performed for picture acquisition.
DatasheetMSDS
The anti-histamine antibody IS1039 is a rabbit polyclonal antibody developed to label Histamine in whole mounts, cell culture and tissue sections. The antibody was validated for IF in mouse and crayfish brain tissues, using the STAINperfect immunostaining kit A.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µl (approx. 40 tissue sections) |
Product overview
| Product name | Histamine antibody – Rabbit pAb |
| Synonyms | Anti-Histamine antibody Anti-2-(4-Imidazolyl)ethylamine antibody |
| Immunogen | Conjugated Histamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Histamine antibody did not show any significant cross reactivity with Histidine conjugate |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for histamine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
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