Research ELISA kit for Dopamine quantification Research ELISA kit for Dopamine quantification

Research ELISA kit for Dopamine quantification

Dopamine ELISA kit - Research

Ref: BA-E-5300

This research Dopamine ELISA kit enables the ultra-sensitive measurement of Dopamine (DA) in any biological sample. Highly flexible, the assay features a minimal sample volume as low as 1 µl, with a limit of detection at 3.3 pg/ml.

 

Sample type
1 - 750 µl, all biological fluids
Samples/kit 40 samples in duplicate
Sensitivity

3.3 pg/ml

Range 0 / 0.5 - 80 ng/ml
Assay time Extraction, acylation and enzymatic conversion (4 hours), ELISA overnight
Species reactivity Reacts with all species
Citations Over 20
420
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54.00€ , 5 days
Product information Key steps References Technical support Reviews

Product overview

Product name

Dopamine ELISA kit - Research

Description Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Dopamine in biological samples. Research use only.
Format 96-well plate
Samples Any sample
Sample volume 1 - 750 µl
Cross specie-reactivity React with all species
Standard range 0 / 0.5 - 80 ng/ml
Sensitivity

3.3 pg/ml

Specificity No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.
Assay time Sample preparation 4 hours and ELISA overnight
Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for use Material safety datasheet
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Key steps

Sample preparation

Sample extraction and acylation (1h45), enzymatic conversion (2h)

ELISA Dopamine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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References

Product citations


 

 

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Reviews Submit your review

This kit works with microdialysate analysis.
Raphael
We used the Dopamine ELISA kit to measure the dopamine concentration of samples from microdialysis. Even if the kit had never been used on this biological substrate, our pilot experience allowed us to validate the use of this kit on this type of substrate. Finally, for 30uL samples containing about 20nM DA, this kit works well.
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