Research ELISA kit for Dopamine quantification
Dopamine ELISA kit - Research
Ref: BA-E-5300
This research Dopamine ELISA kit enables the ultra-sensitive measurement of Dopamine (DA) in any biological sample. Highly flexible, the assay features a minimal sample volume as low as 1 µl, with a limit of detection at 3.3 pg/ml.
| Sample type |
1 - 750 µl, all biological fluids |
| Samples/kit | 40 samples in duplicate |
| Sensitivity |
3.3 pg/ml |
| Range | 0 / 0.5 - 80 ng/ml |
| Assay time | Extraction, acylation and enzymatic conversion (4 hours), ELISA overnight |
| Species reactivity | Reacts with all species |
| Citations | Over 20 |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Dopamine ELISA kit - Research |
| Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of Dopamine in biological samples. Research use only. |
| Format | 96-well plate |
| Samples | Any sample |
| Sample volume | 1 - 750 µl |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 0.5 - 80 ng/ml |
| Sensitivity |
3.3 pg/ml |
| Specificity | No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | Sample preparation 4 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation |
Sample extraction and acylation (1h45), enzymatic conversion (2h) |
| ELISA | Dopamine antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
Product citations
- Robson MJ et al. σ Receptor antagonist attenuation of methamphetamine-induced neurotoxicity is correlated to body temperature modulation. Pharmacol Rep. 2013;65(2):343-9.
- Seminerio MJ et al. The evaluation of AZ66, an optimized sigma receptor antagonist, against methamphetamine-induced dopaminergic neurotoxicity and memory impairment in mice. Int J Neuropsychopharmacol. 2013 Jun;16(5):1033-44.
- Kim HG et al. Cyperi Rhizoma inhibits the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced reduction in nigrostriatal dopaminergenic neurons in estrogen-deprived mice. J Ethnopharmacol. 2013 Jun 21;148(1):322-8.
- Kaushal N, Seminerio MJ, Robson MJ, McCurdy CR, Matsumoto RR. Pharmacological evaluation of SN79, a sigma (σ) receptor ligand, against methamphetamine-induced neurotoxicity in vivo. Eur Neuropsychopharmacol. 2013 Aug;23(8):960-71.
- Zhang Z, Alexanian AR. Dopaminergic-like cells from epigenetically reprogrammed mesenchymal stem cells. J Cell Mol Med. 2012 Nov;16(11):2708-14.
- Tasset I et al. Neuroprotective effects of extremely low-frequency electromagnetic fields on a Huntington's disease rat model: effects on neurotrophic factors and neuronal density. Neuroscience. 2012 May 3;209:54-63.
- Rönnberg E, Calounova G, Pejler G. Mast cells express tyrosine hydroxylase and store dopamine in a serglycin-dependent manner. Biol Chem. 2012 Jan;393(1-2):107-12.
- ...
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