GABA labeling (green) in mouse cortical primary neurons
Immunodetection of GABA- (green) and MAP2- (red) positive neurons in mouse primary cortical culture. GABAergic neurons were stained using IS1006 anti-GABA antibody combined with MAP2 antibody using the stainperfect immunostaining kit A and appropriate protocol, respectively. Alexa fluor 488 and 546 secondary antibodies were used and pictures were captured by high-content imaging.
GABA antibody - Rabbit polyclonal AbRef: IS1006
The anti-GABA antibody IS1006 rabbit polyclonal antibody displays high affinity and specificity and allows direct detection of GABA in whole mounts, cell culture and tissue sections when samples are prepared using the STAINperfect immunostaining kit A.
|Species reactivity||Reacts with all species
|Tested samples||whole mounts, cell culture, tissue sections|
|Staining procedure||STAINperfect immunostaining kit A|
|Format||50µL (approx. 40 tissue sections)
|References||Citations in literature|
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
GABA antibody - Rabbit Polyclonal antibody
Anti-Gamma-Aminobutyric acid antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with Gamma-Aminobutyric acid analogs, including Beta-Alanine and D-Alanine.|
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
|IF - Cell cultures, Whole mounts, Tissue sections||
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for GABA staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
- Villamayor et al. Structural, morphometric and immunohistochemical study of the rabbit accessory olfactory bulb. Brain Struct Funct. 2019 Dec 4.
- Smits et al., Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids. bioRxiv. 2019, March 28.
- Yokoi et al. Impact of Sleep–Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons Front Neurosci. 2019 May 29.
- Traub et al. hiPS Cell-Derived Neurons for High-Throughput Screening. Methods Mol Biol. 2019;1994:243-263
Selected articles on GABA:
- Liu X, Wang Q, Haydar TF, Bordey A. Nonsynaptic GABA signaling in postnatal subventricular zone controls proliferation of GFAP-expressing progenitors. Nat Neurosci. 2005 Sep;8(9):1179-87. Epub 2005 Aug 14.
- Lawrence JJ. Cholinergic control of GABA release: emerging parallels between neocortex and hippocampus. Trends Neurosci. 2008 Jul;31(7):317-27. doi: 10.1016/j.tins.2008.03.008. Epub 2008 Jun 13.
- Baulac S, Huberfeld G, Gourfinkel-An I, Mitropoulou G, Beranger A, Prud'homme JF, Baulac M, Brice A, Bruzzone R, LeGuern E. First genetic evidence of GABA(A) receptor dysfunction in epilepsy: a mutation in the gamma2-subunit gene. Nat Genet. 2001 May;28(1):46-8.