L-Glutamate and GABA in adult mouse primary cortical neurons
Adult mouse primary cortical neurons were stained with mouse monoclonal anti-L-glutamate antibody (red) combined with anti-GABA rabbit polyclonal antibody (green). Staining was performed using optimized sample preparation with STAINperfect immunostaining kit A and according to the protocol for cell culture. Fluorescent labeled secondary antibody were used and pictures were acquired by confocal imaging.
L-Glutamate antibody - mouse monoclonal AbRef: IS018
Anti-L-glutamate antibody 1D5-1B1 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels L-Glutamate in whole mounts, cell culture and tissue sections.
|Applications||IHC / IF
|Species reactivity||Reacts with all species|
|Tested samples||whole mounts, cell culture, tissue sections
|Staining procedure||STAINperfect immunostaining kit A|
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
L-Glutamate antibody - mouse mAb
Anti-L-Glutamic acid antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated Glutamate antibody did not show any significant cross reactivity with its analogs, including D-Glutamate.
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
|IF - Cell cultures, Whole mounts, Tissue sections||
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for L-Glutamate staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
Antibody not yet cited. Submit an article and get a 10% discount.
Selected articles about L-Glutamate:
- Okubo Y et al. Imaging extrasynaptic glutamate dynamics in the brain. Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6526-31.
- Meldrum BS. Glutamate as a neurotransmitter in the brain: review of physiology and pathology. J Nutr. 2000 Apr;130(4S Suppl):1007S-15S.
- Hynd MR, Scott HL, Dodd PR. Glutamate-mediated excitotoxicity and neurodegeneration in Alzheimer's disease. Neurochem Int. 2004 Oct;45(5):583-95.