L-Glutamate and GABA in adult mouse primary cortical neurons
Adult mouse primary cortical neurons were stained with mouse monoclonal anti-L-glutamate antibody (red) combined with anti-GABA rabbit polyclonal antibody (green). Staining was performed using optimized sample preparation with STAINperfect immunostaining kit A and according to the protocol for cell culture. Fluorescent labeled secondary antibody were used and pictures were acquired by confocal imaging.
L-Glutamate antibody - mouse monoclonal Ab
Ref: IS018
Anti-L-glutamate antibody 1D5-1B1 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels L-Glutamate in whole mounts, cell culture and tissue sections.
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Applications | IHC / IF |
| Species reactivity | Reacts with all species |
| Tested samples | whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name |
L-Glutamate antibody - mouse mAb |
| Synonyms |
Anti-L-Glutamic acid antibody |
| Immunogen | Conjugated L-Glutamate |
| Specificity | When tested in competitive ELISA, the anti-conjugated Glutamate antibody did not show any significant cross reactivity with its analogs, including D-Glutamate. |
| Volume | 50µL |
| Lot number |
140313 |
Storage
| Form |
Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| IF - Cell cultures, Whole mounts, Tissue sections |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for L-Glutamate staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
References
Antibody not yet cited. Submit an article and get a 10% discount.
Selected articles about L-Glutamate:
- Okubo Y et al. Imaging extrasynaptic glutamate dynamics in the brain. Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6526-31.
- Meldrum BS. Glutamate as a neurotransmitter in the brain: review of physiology and pathology. J Nutr. 2000 Apr;130(4S Suppl):1007S-15S.
- Hynd MR, Scott HL, Dodd PR. Glutamate-mediated excitotoxicity and neurodegeneration in Alzheimer's disease. Neurochem Int. 2004 Oct;45(5):583-95.
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