L-Glutamate and GABA in adult mouse primary cortical neurons L-Glutamate and GABA in adult mouse primary cortical neurons Staining of glutamatergic neurons in mouse primary cortical culture Affinity & specificity of anti-L-Glutamate monoclonal antibody 1D5-1B1 L-Glutamate (L-Glu)

L-Glutamate and GABA in adult mouse primary cortical neurons

Adult mouse primary cortical neurons were stained with mouse monoclonal anti-L-glutamate antibody (red) combined with anti-GABA rabbit polyclonal antibody (green). Staining was performed using optimized sample preparation with STAINperfect immunostaining kit A and according to the protocol for cell culture. Fluorescent labeled secondary antibody were used and pictures were acquired by confocal imaging.

L-Glutamate antibody - mouse monoclonal Ab

Ref: IS018


Anti-L-glutamate antibody 1D5-1B1 is a mouse monoclonal Ab specifically selected by competitive ELISA for its affinity and specificity features. Using with the STAINperfect immunostaining kit A, our antibody directly labels L-Glutamate in whole mounts, cell culture and tissue sections.

 

Clonality Monoclonal antibody
Host Mouse
Applications IHCIF
Species reactivity Reacts with all species
Tested samples whole mounts, cell culture, tissue sections
Staining procedure STAINperfect immunostaining kit A
Format 50µL
440
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Product overview


 

Product name

L-Glutamate antibody - mouse mAb

Synonyms

Anti-L-Glutamic acid antibody

Immunogen Conjugated L-Glutamate
Specificity When tested in competitive ELISA, the anti-conjugated Glutamate antibody did not show any significant cross reactivity with its analogs, including D-Glutamate.
Volume 50µL
Lot number

140313

 

 

Storage


 

Form

Liquid

Purity Purified anti-serum
Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

IF - Cell cultures, Whole mounts, Tissue sections

Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol for L-Glutamate staining
Protocols-at-a-glance

 

 

 

STAINperfect neurotransmitters instructions for use

STAINperfect neurotransmitter staining protocol cell cultures STAINperfect neurotransmitter staining protocol whole mounts STAINperfect neurotransmitter staining protocol tissue sections
Complete 
Instructions for Use
Protocol-at-a-glance 
for cell cultures
Protocol-at-a-glance 
for whole mounts
Protocol-at-a-glance 
for tissue sections


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